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Abstract：
This study aimed to elucidate the role of PDIA3 in visceral hypersensitivity of rats with irritable bowel syndrome (IBS). Fourty eight SD rats were randomly divided into four groups (n=12): control group, IBS-empty virus group (IBS-1), IBS-PDIA3 silence group (IBS-2), and IBS-the control group (IBS-3). Visceral hypersensitivity models were established by using acetic acid enema combined with restraint stress, and assayed by abdominal withdrawal reflexes (AWR). Mast cells (MCs) in ileocecal mucosa were counted with toluidine blue staining. Degranulation of MCs was observed under electron microscopy. Serum and mucosal levels of IL-4 and IL-9 were measured with ELISA and QT-PCR. Intestinal tryptase and PAR-2 expression was examined with ELISA and Western blot. The Results showed that PDIA3 plays an important role in the formation of visceral hypersensitivity by increasing systemic and colon mucosal expressions of IL-4 and IL-9, activating mast cells and upregulating PAR-2 expression of target organs.
摘要：
本研究旨在阐明PDIA3在肠易激综合征（IBS）大鼠内脏高敏感性中的作用。将48只SD大鼠随机分为4组(n=12):对照组,IBS-空病毒组(IBS-1),IBS-PDIA3静默组(IBS-2),和IBS-对照组(IBS-3)。采用醋酸灌肠结合束缚应激建立内脏超敏反应模型,并通过腹部退缩反射（AWR）进行分析。用甲苯胺蓝染色计数回盲肠粘膜中的肥大细胞（MC）。在电子显微镜下观察到MC的脱粒。ELISA和QT-PCR检测血清和黏膜IL-4和IL-9水平。用ELISA和Westernblot检测肠道类胰蛋白酶和PAR-2的表达。结果表明，PDIA3通过增加全身和结肠黏膜IL-4和IL-9的表达，激活肥大细胞并上调靶器官PAR-2的表达，在内脏超敏反应的形成中起重要作用。
