关键词: Barley Gluten Improving mechanism β-Glucan β-Glucanase

Mesh : Bread / standards Fermentation Flour / standards Food Handling Glutens / chemistry Glycoside Hydrolases / metabolism Hordeum / chemistry Hydrolysis Microscopy, Electron, Scanning Molecular Weight Rheology Spectroscopy, Fourier Transform Infrared Spectrum Analysis, Raman Viscosity beta-Glucans / chemistry

来  源:   DOI:10.1016/j.foodchem.2019.126026   PDF(Sci-hub)

Abstract:
The enrichment of β-glucan in barley significantly decreases the quality of dough and baked bread, and β-glucanase can improve dough and bread quality. Nevertheless, the mechanism by which β-glucanase improves the quality of fermented barley flour-based products is still poorly understood. The gluten microcosmic structure, molecular structure and yeast gas production capacity were investigated using Fourier transform infrared (FT-IR) spectroscopy, Raman spectroscopy, Scanning electron microscopy (SEM) and F3 rheological fermentation techniques. The results showed that β-Glucanase can degrade the high-molecular-weight β-glucan to low-molecular-weight oligosaccharide fragments, which reduces the viscosity of the β-glucans, promotes cross-linking between the gluten molecules, and indirectly improves the gluten network structure, thereby alleviating the negative effects of β-glucans. The β-glucosidase produced during yeast metabolism can further hydrolyse low-molecular-weight oligosaccharides into reducing sugars that can be used by yeast, increasing the carbon sources available to yeasts and the gas production capability of yeasts.
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