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Abstract：
This proceedings report presents the outcomes from an international expert meeting to establish consensus guidelines on IVF culture conditions. Topics reviewed and discussed were: embryo culture - basic principles and interactions; temperature in the IVF laboratory; humidity in culture; carbon dioxide control and medium pH; oxygen tension for embryo culture; workstations - design and engineering; incubators - maintaining the culture environment; micromanipulation - maintaining a steady physcochemical environment; handling practices; assessment practices; culture media - buffering and pH, general composition and protein supplementation, sequential or single-step media for human embryo culture; use and management - cold chain and storage; test equipment - calibration and certification; and laboratory equipment and real-time monitoring. More than 50 consensus guideline points were established under these general headings.
摘要：
本会议报告介绍了国际专家会议的结果，以建立有关IVF培养条件的共识指南。审查和讨论的主题是：胚胎培养-基本原理和相互作用；IVF实验室的温度；培养中的湿度；二氧化碳控制和中等pH值；胚胎培养的氧气张力；工作站-设计和工程；孵化器-维持培养环境；显微操作-维持稳定的物理化学环境；处理实践；评估实践；培养基-缓冲和pH值，一般成分和蛋白质补充剂，用于人类胚胎培养的顺序或单步培养基；使用和管理-冷链和储存；测试设备-校准和认证；以及实验室设备和实时监控。在这些一般标题下建立了50多个共识准则点。
