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Abstract：
Serum iron concentration is usually decreased in true iron deficiency and with inflammatory disease in man and domestic animals. Serum total iron binding capacity (TIBC) may be increased in true iron deficiency and decreased with inflammatory disease. This prospective study was designed to measure serum iron analytes in healthy free-ranging and housed Florida manatees (Trichechus manatus latirostris) of both sexes and various ages and to evaluate the effects of diseases common to manatees on these analytes. Blood samples were collected without anticoagulant from 137 healthy free-ranging manatees, 90 healthy housed manatees and 74 free-ranging diseased manatees, and serum was prepared by centrifugation. Serum iron concentration and unsaturated iron binding capacity were measured colourimetrically, and TIBC and percent transferrin saturation with iron were calculated. Serum amyloid A (SAA) was measured to assist in the health assessment of manatees and provide evidence of inflammation in diseased manatees. Based on the serum iron analytes, iron availability was lower in immature manatees compared with adults, and it was lower in housed manatees compared with free-ranging manatees. In contrast to other mammals studied, serum iron concentration was elevated rather than depressed in late pregnancy. Serum iron concentrations and transferrin saturation with iron percentages were significantly lower, and SAA concentrations were significantly higher, in diseased (ill and injured) manatees compared with healthy manatees. Serum iron concentration and transferrin saturation with iron values were negatively correlated with SAA concentrations, and manatees with the highest SAA concentrations had lower serum TIBC values. These findings indicate that inflammation is the major factor responsible for alterations in iron analytes in diseased manatees. Consequently, hypoferraemia may be used as supportive evidence of inflammatory disease in manatees (unless haemorrhage is also present). A decision threshold of ≤13.8 μmol/l was determined for hypoferraemia using receiver operating curve analysis. Based on studies in man and domestic animals, iron therapy is unnecessary for manatees with hypoferraemia associated with inflammation and has the potential for causing tissue damage and increased susceptibility to bacterial infections.
摘要：
在人和家畜中，真正的铁缺乏和炎症性疾病时，血清铁浓度通常会降低。血清总铁结合力（TIBC）可能在真性铁缺乏时增加，而在炎症性疾病中降低。这项前瞻性研究旨在测量健康的自由放养和居住在佛罗里达海牛（Trichechusmanatuslatirostris）中的血清铁分析物，并评估海牛常见疾病对这些分析物的影响。从137只健康的自由放养海牛中收集了不含抗凝剂的血液样本，90只健康的海牛和74只自由放养的患病海牛，离心制备血清。用比色法测量血清铁浓度和不饱和铁结合能力，计算TIBC和含铁的转铁蛋白饱和度。测量血清淀粉样蛋白A（SAA）以帮助海牛的健康评估，并提供患病海牛炎症的证据。基于血清铁分析物，与成年海牛相比，未成熟海牛的铁利用率较低，与自由放养的海牛相比，居住的海牛的数量较低。与其他研究的哺乳动物相比，妊娠晚期血清铁浓度升高而不是降低。血清铁浓度和转铁蛋白饱和度随铁百分比显著降低，SAA浓度明显更高，与健康海牛相比，患病（生病和受伤）海牛。血清铁浓度和转铁蛋白饱和度与铁值呈负相关，与SAA浓度呈负相关，SAA浓度最高的海牛血清TIBC值较低。这些发现表明炎症是导致患病海牛中铁分析物改变的主要因素。因此,低铁血症可作为海牛炎症性疾病的支持性证据(除非同时存在出血).使用受试者工作曲线分析确定的低铁血症的决策阈值≤13.8μmol/l。根据对人类和家畜的研究，铁治疗对于与炎症相关的低铁血症海牛是不必要的，并且有可能导致组织损伤和增加对细菌感染的敏感性。
