Development of a biochemical and biophysical suite for integral membrane protein targets: A review.

用于整合膜蛋白靶标的生化和生物物理套件的开发 ： 综述。

• 影响指数: 2.025
• 发表时间: 03 2020
• 来源期刊: Protein Expr Purif
• DOI: 10.1016/j.pep.2019.105545
• PMID: 31778786
• 文章类型: Journal Article
• 中科院分区: Q4
• 方向: 生物
• 作者列表: Haffke M,Duckely M,Bergsdorf C,Jaakola VP,Shrestha B

关键词: Expression; G protein coupled receptors; GPCR; Insect cells; Integral membrane proteins; Mammalian cells

Mesh: Animals; Antibodies; Baculoviridae / genetics; Biophysics; Cell Line; Drug Design; Drug Industry; Gene Expression; Humans; Insecta / genetics; Mammals / genetics; Membrane Proteins / biosynthesis chemistry immunology isolation & purification; Receptors, G-Protein-Coupled / biosynthesis chemistry immunology isolation & purification; Recombinant Proteins / biosynthesis chemistry immunology isolation & purification

Abstract

The generation of integral membrane proteins (IMPs) in heterologous systems and their characterization remains a major challenge in biomedical research. Significant efforts have been invested both in academia and in the pharmaceutical industry to establish technologies for the expression, isolation and characterization of IMPs. Here we summarize some of the key aspects, which are important to support structure-based drug design (SBDD) in drug discovery projects. We furthermore include timeline estimates and an overview of the target selection and biophysical screening approaches.

摘要

异源系统中整合膜蛋白（IMP）的产生及其表征仍然是生物医学研究中的主要挑战。学术界和制药业都投入了大量努力来建立表达的技术，IMP的分离和表征。在这里，我们总结了一些关键方面，这对于在药物发现项目中支持基于结构的药物设计（SBDD）很重要。我们还包括时间表估计和目标选择和生物物理筛选方法的概述。