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Abstract：
Microbial communities interplay with their environment through their functional traits that can be a response or an effect on the environment. Here, we explore how a functional trait-the decomposition of organic matter, can be addressed based on genetic markers and how the expression of these markers reflect ecological strategies of two fungal litter decomposer Gymnopus androsaceus and Chalara longipes. We sequenced the genomes of these two fungi, as well as their transcriptomes at different steps of Pinus sylvestris needles decomposition in microcosms. Our results highlighted that if the gene content of the two species could indicate similar potential decomposition abilities, the expression levels of specific gene families belonging to the glycoside hydrolase category reflected contrasting ecological strategies. Actually, C. longipes, the weaker decomposer in this experiment, turned out to have a high content of genes involved in cell wall polysaccharides decomposition but low expression levels, reflecting a versatile ecology compare to the more competitive G. androsaceus with high expression levels of keystone functional genes. Thus, we established that sequential expression of genes coding for different components of the decomposer machinery indicated adaptation to chemical changes in the substrate as decomposition progressed.
摘要：
微生物群落通过其功能特性与环境相互作用，这些特性可能是对环境的反应或影响。这里,我们探索了一种功能特征——有机物的分解，可以根据遗传标记以及这些标记的表达如何反映两种真菌凋落物分解剂Gymnopusandrosacus和Chalaralongipes的生态策略来解决。我们对这两种真菌的基因组进行了测序，以及它们在微观世界中樟子松针叶分解的不同步骤的转录组。我们的结果强调，如果两个物种的基因含量可以表明相似的潜在分解能力，属于糖苷水解酶类别的特定基因家族的表达水平反映了相反的生态策略。事实上,C.longipes,这个实验中较弱的分解器，结果发现，参与细胞壁多糖分解的基因含量高，但表达水平低，与具有高表达水平的梯形功能性基因的更具竞争性的G.androsacus相比，反映了一种通用的生态学。因此，我们确定，编码分解器机制不同成分的基因的顺序表达表明，随着分解的进行，对底物化学变化的适应。
