In Vivo and In Vitro Study of Immunostimulation by Leuconostoc lactis-Produced Gluco-Oligosaccharides.

乳酸明串珠菌产生的葡萄糖 - 寡糖的免疫刺激的体内和体外研究。

• 影响指数: 4.927
• 发表时间: Nov 2019 5
• 来源期刊: Molecules
• DOI: 10.3390/molecules24213994
• PMID: 31694180
• 文章类型: Journal Article
• 中科院分区: Q3
• 作者列表: Lee S,Song IH,Park YS

关键词: MAPK signaling pathway; cyclophosphamide; immunostimulation; oligosaccharides; phagocytosis

Mesh: Animals; Cell Line; Extracellular Signal-Regulated MAP Kinases / metabolism; Immunization / methods; Interleukin-10 / metabolism; Interleukin-6 / metabolism; JNK Mitogen-Activated Protein Kinases / metabolism; Leuconostoc / metabolism; Macrophages / drug effects metabolism; Male; Mice; Mice, Inbred BALB C; Oligosaccharides / pharmacology; Phagocytosis / drug effects; Phosphorylation / drug effects; RAW 264.7 Cells; Signal Transduction / drug effects; Tumor Necrosis Factor-alpha / metabolism; Up-Regulation / drug effects; p38 Mitogen-Activated Protein Kinases / metabolism

Abstract

Glycosyltransferase-producing Leuconostoc lactis CCK940 produces CCK- oligosaccharides, gluco-oligosaccharide molecules, using sucrose and maltose as donor and acceptor molecules, respectively. In this study, the immunostimulatory activities of CCK-oligosaccharides on RAW264.7 macrophages and BALB/c mice were evaluated. CCK-oligosaccharides induced the expression of phosphorylated-p38, extracellular-signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK) and upregulation of phagocytic activity in RAW264.7 macrophages, suggesting their involvement in mitogen-activated protein kinase (MAPK) signaling pathway and phagocytosis. When CCK-oligosaccharides were administered to mice intraperitoneally injected with cyclophosphamide (CY), spleen indices and expressions of interleukin (IL)-6, IL-10, and tumor necrosis factor-α increased, compared with those in only CY-treated group. These findings suggest that CCK-oligosaccharides can be used as an effective immunostimulating agent.

摘要

产生糖基转移酶的乳酸明串珠菌CCK940产生CCK-寡糖，葡糖寡糖分子，使用蔗糖和麦芽糖作为供体和受体分子，分别。在这项研究中,评估CCK寡糖对RAW264.7巨噬细胞和BALB/c小鼠的免疫刺激活性。CCK寡糖诱导磷酸化p38的表达，细胞外信号调节激酶（ERK），和c-JunN末端激酶（JNK）和上调RAW264.7巨噬细胞的吞噬活性，提示它们参与丝裂原活化蛋白激酶（MAPK）信号通路和吞噬作用。当CCK寡糖给小鼠腹腔注射环磷酰胺（CY）时，脾脏指数和白细胞介素（IL）-6，IL-10和肿瘤坏死因子-α的表达增加，与仅CY治疗组相比。这些发现表明CCK-寡糖可用作有效的免疫刺激剂。