A novel fluorescent chemosensor for detection of mercury(II) ions based on dansyl-peptide and its application in real water samples and living LNcap cells.

• 影响指数: 4.831
• 发表时间: Feb 2020 5
• 来源期刊: Spectrochim Acta A Mol Biomol Spectrosc
• DOI: 10.1016/j.saa.2019.117616
• PMID: 31605969
• 文章类型: Journal Article
• 作者列表: Xue S,Wang P,Chen K

关键词: Cells imaging; Chemosensor; Hg(II) ions; Peptide; Real water samples

Mesh: Biosensing Techniques / methods; Cell Line, Tumor; Dansyl Compounds / chemistry; Fluorescent Dyes / chemical synthesis chemistry pharmacology; Humans; Ions / analysis; Limit of Detection; Materials Testing; Mercury / analysis; Peptides / chemistry; Spectrometry, Fluorescence / methods; Waste Water / analysis; Water Pollutants / analysis

Abstract

Mercury is one of the most hazardous pollutants, and mercury pollution is a serious hazard to our environment. Herein, we designed and synthesized a new peptide-based fluorescent chemosensor (L) based on a Fmoc-Lys (Fmoc)-OH backbone conjugated with two Serines and dansyl groups using solid phase peptide synthesis (SPPS) technology. L exhibited highly selective and excellent sensitive detection of Hg2+ ions in 100% aqueous solutions through fluorescence quenching. The chemosensor L forms a 2:1 stoichiometry with high binding constants (4.89×106M-1) and the detection limit for Hg2+ ions of the proposed assay was 7.59nM. In addition, the recovery test results of Hg2+ concentration in actual water samples showed that the quantitative detection of Hg2+ ions can be realized in two water samples. Moreover, L showed low cytotoxicity and excellent membrane permeability in HK2 cells, which has been successfully applied for monitoring Hg2+ ions in living LNCaP cells.