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Abstract：
The use of blood-based eye drops as therapy for various diseases of the ocular surface has become increasingly popular in ophthalmic practice during recent years. The rationale for their use is based on the promotion of cellular proliferation and migration thanks to the supply of metabolically active substances, in particular growth factors. Blood-derived eye drops have been used for the treatment of several ocular surface disorders, such as dry eye disease, corneal ulcer, persistent epithelial defect, neurotrophic keratitis, ocular surface burn, recurrent corneal erosion, and limbal stem-cell deficiency. Both autologous (from patients themselves) and heterologous (from adult donors or from cord blood sampled at birth)-derived products exist, and each source has specific pros and cons. Despite an extensive literature, several issues are still under debate and the aim of this manuscript is to review the indications, preparation methods and storage, characterization of content, rationale for clinical outcomes, patient stratification, length of treatment, and rationale for repeated treatments at disease relapse. A rationale based on a \"5 Ws and 2 Hs\" protocol is proposed as a way of thinking, with the attempt to clarify Who, Why, When, Where, What, and How to use these treatment options.
摘要：
近年来,使用基于血液的滴眼剂作为各种眼表疾病的疗法在眼科实践中变得越来越流行。其使用的理由是基于代谢活性物质的供应促进细胞增殖和迁移，特别是增长因素。血液衍生的滴眼液已用于治疗几种眼表疾病，如干眼症，角膜溃疡,持续性上皮缺损,神经营养性角膜炎，眼表烧伤,复发性角膜糜烂，角膜缘干细胞缺乏症.自体（来自患者本身）和异源（来自成年捐献者或来自出生时的脐带血采样）来源的产品都存在，每个来源都有特定的利弊。尽管有大量的文献，几个问题仍在辩论中，本手稿的目的是审查适应症，制备方法和储存，内容的表征，临床结果的基本原理，患者分层,治疗时间，以及疾病复发时重复治疗的理由。提出了一种基于“5Ws和2Hs”协议的基本原理，作为一种思考方式，试图澄清是谁，为什么,When,Where,什么,以及如何使用这些治疗方案。
