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Abstract：
Objectives: Long noncoding RNA (lncRNA) SBF2-AS1 was found to be related to some tumors. Nevertheless, the role of SBF2-AS1 in osteosarcoma (OS) is still needed to be elaborated. This study is conducted to examine the expression of lncRNA SBF2-AS1 in OS with the involvement of microRNA-30a (miR-30a) and FOXA1. Methods: OS tissues and its corresponding adjacent normal tissues were obtained for the detection of SBF2-AS1 expression and its relations with clinical phenotypes. OS cells with most significant expression of SBF2-AS1 were selected for subsequent experiments. Moreover, a series of experiments were performed to detect proliferation, invasion, migration, colony formation, cell cycle distribution and apoptosis of OS cells. Furthermore, the binding site between SBF2-AS1 and miR-30a as well as between miR-30a and FOXA1 was verified. Results: SBF2-AS1 was overexpressed in tissues and cells of OS. Additionally, silencing of SBF2-AS1 and miR-30a overexpression inhibited the proliferation, migration and invasion of OS cells and promoted their apoptosis. Moreover, lncRNA SBF2-AS1 regulated miR-30a by serving as a ceRNA, thus promoting FOXA1 expression. Furthermore, interfered SBF2-AS1 or upregulated miR-30a restrained the growth of OS. Conclusion: Our study confirms that silencing of SBF2-AS1 represses proliferation, migration and invasion of OS cells and promotes their apoptosis by binding to miR-30a and inhibiting FOXA1 expression.
摘要：
目的：发现长链非编码RNA（lncRNA）SBF2-AS1与某些肿瘤有关。然而，SBF2-AS1在骨肉瘤(OS)中的作用仍需进一步阐述.进行这项研究以检查OS中lncRNASBF2-AS1的表达，并涉及microRNA-30a（miR-30a）和FOXA1。方法：获取OS组织及其相应的癌旁正常组织，检测SBF2-AS1的表达及其与临床表型的关系。选择具有最显著的SBF2-AS1表达的OS细胞用于随后的实验。此外，进行了一系列实验来检测增殖，入侵,迁移,菌落形成,OS细胞的细胞周期分布和凋亡。此外，验证了SBF2-AS1与miR-30a之间以及miR-30a与FOXA1之间的结合位点.结果：SBF2-AS1在OS的组织和细胞中过表达。此外,沉默的SBF2-AS1和miR-30a过表达抑制增殖,OS细胞的迁移和侵袭并促进其凋亡。此外，lncRNASBF2-AS1通过充当CERNA来调节miR-30a，从而促进FOXA1表达。此外，干扰SBF2-AS1或上调miR-30a抑制OS的生长。结论：我们的研究证实SBF2-AS1的沉默抑制增殖，通过与miR-30a结合并抑制FOXA1表达,促进OS细胞的迁移和侵袭,促进其凋亡。
