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Abstract：
Excessive expression of TNF-α worsens bacterial vaginosis (BV) and osteoporosis. Therefore, to understand whether probiotics could alleviate vaginosis and osteoporosis, we isolated anti-inflammatory Lactobacillus plantarum NK3 and Bifidobacterium longum NK49 from kimchi and human fecal lactic acid bacteria collection and examined their effects on Gardnerella vaginalis (GV)-induced vaginosis and ovariectomy-induced osteoporosis in female mice. Oral gavage of NK3 and/or NK49 significantly alleviated GV-induced vaginosis; these inhibited NF-κB activation and TNF-α expression in the vagina and uterus, and decreased the GV population in the vagina. Furthermore, treatment with NK3 and/or NK49 alleviated ovariectomy-induced osteoporosis and obesity; these increased blood calcium, phosphorus, and osteocalcin levels and suppressed body weight. GV-induced vaginosis and ovariectomy increased colonic myeloperoxidase activity, TNF-α expression, and fecal Proteobacteria population. NK3 and/or NK49 treatments reduced TNF-α expression and NF-κB activation in the colon. NK3 and NK49 treatment also restored GV- or ovariectomy-disrupted gut microbiota composition. In conclusion, NK3 and NK49 may simultaneously alleviate BV and osteoporosis by suppressing NF-κB-linked TNF-α expression through the regulation of gut microbiota population.
摘要：
TNF-α的过度表达会加重细菌性阴道病（BV）和骨质疏松症。因此,了解益生菌是否能缓解阴道病和骨质疏松症，我们从泡菜和人类粪便乳酸菌中分离了抗炎植物乳杆菌NK3和长双歧杆菌NK49，并研究了它们对阴道加德纳菌（GV）诱导的阴道病和卵巢切除术诱导的雌性小鼠骨质疏松症的影响。口服NK3和/或NK49可显着减轻GV诱导的阴道病；这些抑制阴道和子宫中NF-κB激活和TNF-α表达，并减少了阴道中的GV数量。此外，用NK3和/或NK49治疗减轻卵巢切除术引起的骨质疏松症和肥胖;这些增加的血钙,磷，和骨钙蛋白水平和抑制体重。GV引起的阴道病和卵巢切除术增加了结肠髓过氧化物酶活性，TNF-α表达,和粪便变形菌种群。NK3和/或NK49治疗降低了结肠中的TNF-α表达和NF-κB活化。NK3和NK49治疗也恢复了GV或卵巢切除术破坏的肠道微生物群组成。总之,NK3和NK49可能通过调节肠道微生物群来抑制NF-κB相关的TNF-α表达，从而同时减轻BV和骨质疏松症。
