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Abstract：
OBJECTIVE: We evaluated the performance of anti-Müllerian hormone (AMH) measured by the Beckman Coulter fully automated Access assay to predict oocyte yield following controlled ovarian stimulation (COS) for in vitro fertilization (IVF).
METHODS: The correlation between the Access assay and the pre-mixing method with Generation II ELISA assay (Gen II pre-mix assay) was assessed using 230 blood samples. The relationship of AMH level measured by the Access assay and the actual number of oocytes retrieved following COS was assessed using 3296 IVF cycles. The performances of AMH, follicle stimulating hormone (FSH), and estradiol (E2) in predicting the responses to COS were also evaluated by constructing receiver operating characteristic (ROC) curves.
RESULTS: The AMH levels measured just before oocyte retrieval by the Access assay and the number of oocytes retrieved following COS showed a good correlation with R = 0.655. The ROC analysis revealed that the sensitivity of AMH was comparable with or lower than that of E2 but higher than that of FSH.
CONCLUSIONS: With the improved Access AMH assays, AMH was as sensitive as E2 and could become an accurate marker of ovarian response to COS in more than 3000 Japanese IVF patients.
METHODS: The correlation between the Access assay and the pre-mixing method with Generation II ELISA assay (Gen II pre-mix assay) was assessed using 230 blood samples. The relationship of AMH level measured by the Access assay and the actual number of oocytes retrieved following COS was assessed using 3296 IVF cycles. The performances of AMH, follicle stimulating hormone (FSH), and estradiol (E2) in predicting the responses to COS were also evaluated by constructing receiver operating characteristic (ROC) curves.
RESULTS: The AMH levels measured just before oocyte retrieval by the Access assay and the number of oocytes retrieved following COS showed a good correlation with R = 0.655. The ROC analysis revealed that the sensitivity of AMH was comparable with or lower than that of E2 but higher than that of FSH.
CONCLUSIONS: With the improved Access AMH assays, AMH was as sensitive as E2 and could become an accurate marker of ovarian response to COS in more than 3000 Japanese IVF patients.
摘要：
目的：我们评估了通过BeckmanCoulter全自动Access测定法测量的抗苗勒管激素（AMH）的性能，以预测体外受精（IVF）的控制性卵巢刺激（COS）后的卵母细胞产量。
方法：使用230份血液样品评估了Access测定与II代ELISA测定（II代预混测定）的预混合方法之间的相关性。使用3296个IVF周期评估了通过Access测定测量的AMH水平与COS后回收的卵母细胞的实际数量之间的关系。AMH的表演，卵泡刺激素（FSH），还通过构建受试者工作特征（ROC）曲线来评估雌二醇（E2）在预测对COS的反应中的作用。
结果：在取卵前通过Access测定测得的AMH水平和COS后取卵的数量显示出与R=0.655的良好相关性。ROC分析显示，AMH的敏感性与E2相当或低于E2，但高于FSH。
结论：通过改进的AccessAMH测定，AMH与E2一样敏感，可以成为3000多名日本IVF患者卵巢对COS反应的准确标志物。
方法：使用230份血液样品评估了Access测定与II代ELISA测定（II代预混测定）的预混合方法之间的相关性。使用3296个IVF周期评估了通过Access测定测量的AMH水平与COS后回收的卵母细胞的实际数量之间的关系。AMH的表演，卵泡刺激素（FSH），还通过构建受试者工作特征（ROC）曲线来评估雌二醇（E2）在预测对COS的反应中的作用。
结果：在取卵前通过Access测定测得的AMH水平和COS后取卵的数量显示出与R=0.655的良好相关性。ROC分析显示，AMH的敏感性与E2相当或低于E2，但高于FSH。
结论：通过改进的AccessAMH测定，AMH与E2一样敏感，可以成为3000多名日本IVF患者卵巢对COS反应的准确标志物。
