A pilot study to assess peripheral blood TCR β-chain CDR3 repertoire in occupational medicamentosa-like dermatitis due to trichloroethylene using high-throughput sequencing.

一项使用高通量测序评估三氯乙烯所致职业性药疹样皮炎外周血 TCR β 链 CDR3 库的初步研究。

• 影响指数: 5.785
• 发表时间: Oct 2019
• 来源期刊: Environ Toxicol Pharmacol
• DOI: 10.1016/j.etap.2019.103211
• PMID: 31212134
• 文章类型: Journal Article
• 中科院分区: Q2
• 方向: 生物
• 作者列表: Lin D,Wang D,Li P,Yang Y,Liu W,Zhang L,Zhang Z,Zhang Y,Huang X

关键词: Complementarity-determining region 3; High-throughput sequencing; Occupational medicamentosa-like dermatitis due to trichloroethylene; T cell receptor repertoire; Trichloroethylene

Mesh: Adult; Case-Control Studies; Complementarity Determining Regions / blood genetics; Drug Eruptions / blood genetics; Female; High-Throughput Nucleotide Sequencing; Humans; Male; Occupational Diseases / blood; Occupational Exposure / adverse effects analysis; Pilot Projects; Receptors, Antigen, T-Cell, alpha-beta / blood genetics; T-Lymphocytes / drug effects; Trichloroethylene / toxicity

Abstract

We exploratively characterized T cell receptor (TCR) repertoires from occupational medicamentosa-like dermatitis due to trichloroethylene (OMDT) patients to better understand the underlying pathological mechanism. We used a combination of multiplex-PCR, Illumina sequencing and IMGT/High V-QUEST to analyze the characteristics and polymorphisms of the TCR β-chain complementarity-determining region 3 (CDR3) gene in 10 OMDT cases and 10 trichloroethylene-exposed healthy tolerant controls. Compared with the tolerant controls, OMDT cases showed no significant difference in TCR repertoire diversity including repertoire breadth, highly expanded clone, and CDR3 length distribution. However, we observed several differences in TRBV/TRBJ usage and combination between the two groups, as well as some shared and unique T cell clones in the cases. The pilot study delineated some features of TCR repertoire in OMDT patients that warrant further investigation.

摘要

我们探索性地表征了由三氯乙烯（OMDT）患者引起的职业性药疹样皮炎的T细胞受体（TCR）库，以更好地了解潜在的病理机制。我们使用了多重PCR的组合，Illumina测序和IMGT/HighV-QUEST分析10例OMDT病例和10例三氯乙烯暴露的健康耐受对照中TCRβ链互补决定区3(CDR3)基因的特征和多态性。与耐受性对照相比，OMDT病例显示TCR库多样性（包括库宽度）没有显着差异，高度扩张的克隆，和CDR3长度分布。然而,我们观察到两组之间在TRBV/TRBJ使用和组合方面的一些差异，以及病例中一些共享和独特的T细胞克隆。初步研究描绘了OMDT患者TCR库的一些特征，需要进一步研究。