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Abstract：
Metabolic MRI is a noninvasive technique that can give new insights into understanding cancer metabolism and finding biomarkers to evaluate or monitor treatment plans. Using this technique, a previous study has shown an increase in pH during neoadjuvant chemotherapy (NAC) treatment, while recent observation in a different study showed a reduced amide proton transfer (APT) signal during NAC treatment (negative relation). These findings are counterintuitive, given the known intrinsic positive relation of APT signal to pH.
In this study we combined APT MRI and 31 P-MRSI measurements to unravel the relation between the APT signal and pH in breast cancer. Twenty-two breast cancer patients were scanned with a 7 T MRI before and after the first cycle of NAC treatment. pH was determined by the chemical shift of inorganic phosphate (Pi).
While APT signals have a positive relation to pH and amide content, we observed a direct negative linear correlation between APT signals and pH in breast tumors in vivo.
As differentiation of cancer stages was confirmed by observation of a linear correlation between cell proliferation marker PE/Pi (phosphoethanolamine over inorganic phosphate) and pH in the tumor, our data demonstrates that the concentration of mobile proteins likely supersedes the contribution of the exchange rate to the APT signal.
In this study we combined APT MRI and 31 P-MRSI measurements to unravel the relation between the APT signal and pH in breast cancer. Twenty-two breast cancer patients were scanned with a 7 T MRI before and after the first cycle of NAC treatment. pH was determined by the chemical shift of inorganic phosphate (Pi).
While APT signals have a positive relation to pH and amide content, we observed a direct negative linear correlation between APT signals and pH in breast tumors in vivo.
As differentiation of cancer stages was confirmed by observation of a linear correlation between cell proliferation marker PE/Pi (phosphoethanolamine over inorganic phosphate) and pH in the tumor, our data demonstrates that the concentration of mobile proteins likely supersedes the contribution of the exchange rate to the APT signal.
摘要：
代谢MRI是一种非侵入性技术，可以为了解癌症代谢和寻找生物标志物以评估或监测治疗计划提供新的见解。使用这种技术，之前的一项研究表明，新辅助化疗(NAC)治疗期间pH值升高，而另一项研究中的最新观察显示，NAC治疗期间酰胺质子转移（APT）信号降低（负相关）。这些发现是违反直觉的,给定已知的APT信号与pH的内在正相关关系。
在这项研究中，我们将APTMRI和31P-MRSI测量相结合，以揭示乳腺癌中APT信号与pH之间的关系。在第一周期NAC治疗前后，对22例乳腺癌患者进行了7TMRI扫描。通过无机磷酸盐(Pi)的化学位移确定pH。
虽然APT信号与pH和酰胺含量呈正相关，我们在体内乳腺肿瘤中观察到APT信号与pH之间存在直接的负线性相关性。
由于肿瘤中细胞增殖标志物PE/Pi（磷酸乙醇胺相对于无机磷酸盐）与pH之间的线性相关的观察，证实了癌症分期的分化。我们的数据表明,移动蛋白的浓度可能取代了汇率对APT信号的贡献.
在这项研究中，我们将APTMRI和31P-MRSI测量相结合，以揭示乳腺癌中APT信号与pH之间的关系。在第一周期NAC治疗前后，对22例乳腺癌患者进行了7TMRI扫描。通过无机磷酸盐(Pi)的化学位移确定pH。
虽然APT信号与pH和酰胺含量呈正相关，我们在体内乳腺肿瘤中观察到APT信号与pH之间存在直接的负线性相关性。
由于肿瘤中细胞增殖标志物PE/Pi（磷酸乙醇胺相对于无机磷酸盐）与pH之间的线性相关的观察，证实了癌症分期的分化。我们的数据表明,移动蛋白的浓度可能取代了汇率对APT信号的贡献.
