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Abstract：
Insects are amongst the greatest pests of agriculture, horticulture and forestry worldwide, inflicting damage and economic costs both directly and by transmitting plant viruses. Many kinds of insects are now resistant or cross-resistant to pesticides. Tracking studies have become very important for combatting insect pests and for better understanding their biology (eg insect population dynamics, movements, feeding behaviour and other ecological interactions). A wide variety of tracing approaches have been used including discriminative, tracer and molecular methods. The perfect technique for insect tracking is the technique that harmonizes with insects\' \'normal\' biology. Furthermore, the technique should be environmentally safe, cost-effective and easy to use. This paper reviews the current techniques used for insect traceability, documents the advantages and drawbacks of each method, and puts special focus on molecular techniques, including PCR-denaturing gradient gel electrophoresis as a new and promising traceability tool that could provide insects with a unique biological barcode and thus make it possible to trace their movements.
摘要：
昆虫是农业最大的害虫之一，世界各地的园艺和林业，直接和通过传播植物病毒造成损害和经济成本。许多种类的昆虫现在对杀虫剂具有抗性或交叉抗性。跟踪研究对于对抗害虫和更好地了解其生物学（例如昆虫种群动态，运动,喂养行为和其他生态相互作用)。已经使用了各种各样的跟踪方法，包括歧视性的，示踪和分子方法。昆虫追踪的完美技术是与昆虫正常生物学相协调的技术。此外，该技术应该是环境安全的，具有成本效益和易于使用。本文回顾了目前用于昆虫溯源的技术，记录每种方法的优缺点，并特别关注分子技术，包括PCR变性梯度凝胶电泳作为一种新的和有前途的可追溯性工具，可以为昆虫提供独特的生物条形码，从而有可能追踪它们的运动。
