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Abstract：
Purpose: Dental unit\'s environment and relevant instruments are a major source of infectious diseases caused by a variety of microorganisms. The application of various disinfectants is one of the most effective methods for reducing or eliminating microbial contamination. The objective of this study was to evaluate the antibacterial effects of deconex and sodium hypochlorite against bacterial taxa isolated from dental unit\'s environment of Ahvaz Jundishapur University of Medical Sciences, southwest of Iran. Methods: In order to evaluate the quality of disinfection, sampling was performed from different parts of 100 clinical units. For bacterial recovery and isolation, samples were enriched and cultured onto different microbiological culture media. Species identification was carried out using phenotypic and molecular methods (16S rDNA sequence analysis). In vitro activity of sodium hypochlorite and deconex were determined by the broth micro-dilution method. Results: According to conventional techniques, Bacillus spp (48%) was the most frequently encountered isolates, followed by staphylococcus spp (26%). By using both techniques, Bacillus subtilis was the most frequently encountered species (n=23, 21%), followed by Bacillus licheniformis (n=8, 7.4%), Streptococcus pneumonia (n=8, 7.4%), Staphylococcus epidermidis (n=8, 7.4%), Staphylococcus saprophyticus (n=8, 7.4%) and Staphylococcus warneri. The highest levels of contamination were observed in oral medications. The deconex had lower minimum inhibitory concentration (MIC) concentration in comparasion to sodium hypochlorite, which showed that deconex was a much more potent disinfectant. Conclusion: In conclusion, the results of the present in vitro study showed that deconex had promising results for decontamination of the tested microorganism, and it is recommended for disinfecting of dental units and environment. In this study, the high percentage of dental unit\'s contamination showed the need to improve disinfection procedures, sterilization systems, and the use of an appropriate concentration of deconex and sodium hypochlorite for dental units decontamination .
摘要：
目的：牙科单位的环境和相关仪器是由各种微生物引起的传染病的主要来源。各种消毒剂的应用是减少或消除微生物污染的最有效方法之一。本研究的目的是评估deconex和次氯酸钠对从AhvazJundishapur医科大学牙科单位环境中分离的细菌类群的抗菌作用，伊朗西南部。方法:为了评价消毒质量,从100个临床单位的不同部位进行采样.细菌回收和分离，将样品富集并培养到不同的微生物培养基上。使用表型和分子方法（16SrDNA序列分析）进行物种鉴定。通过肉汤微量稀释法测定次氯酸钠和deconex的体外活性。结果:根据常规技术,芽孢杆菌属（48％）是最常遇到的分离株，其次是葡萄球菌属(26%)。通过使用这两种技术，枯草芽孢杆菌是最常见的物种（n=23，21％），其次是地衣芽孢杆菌（n=8，7.4%），肺炎链球菌（n=8，7.4%），表皮葡萄球菌（n=8，7.4%），腐生葡萄球菌（n=8，7.4%）和沃氏葡萄球菌。在口服药物中观察到最高水平的污染。与次氯酸钠相比，deconex的最小抑制浓度（MIC）浓度较低，这表明deconex是一种更有效的消毒剂。结论：总之，目前的体外研究结果表明，deconex对被测微生物的去污具有良好的效果，建议对牙科单位和环境进行消毒。在这项研究中,牙科单位的高污染百分比表明需要改进消毒程序，灭菌系统,以及使用适当浓度的deconex和次氯酸钠进行牙科单位去污。
