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Abstract：
The authors report a sensitive electrochemical aptamer-based assay for the cancer biomarker human epidermal growth factor receptor-2 (HER2). It is based on the use of MnO2 nanosheets that were functionalized with phosphate and a HER2 binding aptamer and serve as the electrochemical probe. The assay follows a sandwich protocol. A peptide that can specifically recognize HER2 was immobilized on a gold electrode for the capture of HER2. Then, the functionalized MnO2 nanosheets were linked to the electrode via the binding between HER2 and HER2 aptamer on the MnO2 nanosheets. The reaction of phosphate and aptamer on the MnO2 nanosheets with molybdate leads to the formation of redox-active molybdophosphate. This results in dual signal amplification. The generated electrochemical current was measured at 0.22 V (vs. Ag/AgCl). The assay allows HER2 to be determined in the 0.1 to 500 pg·mL-1 concentration range, and the detection limit is as low as 0.05 pg·mL-1. The assay was successfully applied for the detection of HER2 in spiked human serum samples. Graphical abstract Electrochemical detection of breast cancer biomarker human epidermal growth factor receptor-2 (HER2) is reported utilizing phosphate ions and aptamer functionalized MnO2 nanosheet as probe.
摘要：
作者报告了用于癌症生物标志物人表皮生长因子受体2（HER2）的灵敏的基于电化学适体的测定。它基于用磷酸盐和HER2结合适体官能化并用作电化学探针的MnO2纳米片的使用。该测定遵循夹心方案。可以特异性识别HER2的肽被固定在金电极上以捕获HER2。然后，官能化的MnO2纳米片通过MnO2纳米片上HER2和HER2适体之间的结合连接到电极。MnO2纳米片上的磷酸盐和适体与钼酸盐的反应导致氧化还原活性的钼磷酸盐的形成。这导致双信号放大。在0.22V下测量产生的电化学电流(vs.Ag/AgCl)。该测定法允许在0.1至500pg·mL-1浓度范围内测定HER2，检出限低至0.05pg·mL-1。该方法已成功用于检测加标人血清样品中的HER2。图摘要报道了利用磷酸根离子和适体功能化的MnO2纳米片作为探针的乳腺癌生物标志物人表皮生长因子受体2(HER2)的电化学检测。
