Gene polymorphisms of SFTPB rs7316, rs9752 and PAOX rs1046175 affect the diagnostic value of plasma Pro-SFTPB and DAS in Chinese Han non-small-cell lung cancer patients.

• 影响指数: 4.48
• 发表时间: 09 2019
• 来源期刊: J Cell Biochem
• DOI: 10.1002/jcb.28741
• PMID: 31016788
• 文章类型: Journal Article
• 中科院分区: Q2
• 方向: 生物
• 作者列表: Wang K,Huang Q,Zhao G,Yang J,Yang K,Huang Y

关键词: DAS; NSCLC; ROC curve; pro-SFTPB; single-nucleotide polymorphisms

Mesh: Adenocarcinoma of Lung / blood diagnosis genetics; Adult; Aged; Aged, 80 and over; Biomarkers, Tumor / analysis; Carcinoma, Non-Small-Cell Lung / blood diagnosis genetics; Carcinoma, Squamous Cell / blood diagnosis genetics; Case-Control Studies; Female; Follow-Up Studies; Humans; Lung Neoplasms / blood diagnosis genetics; Lymphatic Metastasis; Male; Middle Aged; Oxidoreductases Acting on CH-NH Group Donors / genetics; Polymorphism, Single Nucleotide; Prognosis; Protein Precursors / blood; Pulmonary Surfactant-Associated Protein B / genetics; Pulmonary Surfactant-Associated Proteins / blood; Risk Factors; Spermine / analogs & derivatives blood

Abstract

Plasma pro-surfactant protein B (pro-SFTPB) and N1,N12-diacetylspermine (DAS) can be used as markers for the diagnosis of non-small-cell lung carcinoma (NSCLC). Whether the genetic diversity affects the application value of Pro-SFTPB and DAS as a diagnostic marker for NSCLC is still unknown. This study aims to explore the relationship between SFTPB rs7316, rs9752 and PAOX rs1046175 gene polymorphisms and the diagnostic value of plasma Pro-SFTPB and DAS in patients with Chinese Han lung cancer. SFTPB rs7316, rs9752 and PAOX rs1046175 genotypes were analyzed by direct sequencing in 425 patients with NSCLC and 425 controls, and the levels of Pro-SFTPB and DAS in plasma were determined by enzyme-linked immunosorbent assay (ELISA). The area under the curve (AUC) of the SFTPB rs7316 locus TT genotype for the diagnosis of NSCLC was 0.758, and the AUC of the TC/CC genotype for the diagnosis of NSCLC was 0.872. The AUC of the SFTPB rs9752 locus GG genotype for the diagnosis of NSCLC was 0.935, and the AUC of the GC/CC genotype for the diagnosis of NSCLC was 0.648. The AUC of the PAOX rs1046175 locus GG for the diagnosis of NSCLC was 0.669, and the AUC of the GC/CC genotype for the diagnosis of NSCLC was 0.749. In conclusion, SFTPB rs7316, rs9752, and PAOX rs1046175 gene polymorphisms affect the diagnostic value of plasma Pro-SFTPB and DAS in patients with Chinese Han NSCLC.