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Abstract：
In this study, a molecularly imprinted polymer capable of recognizing 15 sulfonamides was first synthesized with sulfabenz as the dummy template. The calculation results from computation simulation showed that the specific 3D conformation of the template had an important influence on the polymer\'s recognition ability. Then, the polymer was used as recognition reagent to prepare a chemiluminescence sensor on a conventional 96-well microplate for the determination of the residues of 15 sulfonamides in meat (chicken and pork). Due to the 4-(imidazol-1-yl)phenol-enhanced luminol-H2O2 system, the limits of detection for the 15 analytes were in the range of 1.0-12 pg/mL. The recoveries from the standard fortified blank samples were in the range of 72.7-99%. Furthermore, one assay could be finished within 30 min, and the sensor could be reused 4 times. Therefore, this sensor could be used as a very useful tool for routine screening of residues of sulfonamides in meat samples. Graphical abstract Assay procedures of the molecularly imprinted polymer-based chemiluminescence sensor for determination of sulfonamides.
摘要：
在这项研究中,首先以sulfabenz为虚拟模板合成了能够识别15种磺酰胺的分子印迹聚合物。计算模拟计算结果表明,模板的特定三维构象对聚合物的识别能力有重要影响。然后，该聚合物被用作识别试剂，在常规的96孔微孔板上制备化学发光传感器，用于测定肉类（鸡肉和猪肉）中15种磺胺类药物的残留。由于4-（咪唑-1-基）苯酚增强的鲁米诺-H2O2系统，15种分析物的检测限在1.0-12pg/mL的范围内。标准强化空白样品的回收率在72.7-99％的范围内。此外，一个测定可以在30分钟内完成，传感器可以重复使用4次。因此,该传感器可用作常规筛查肉类样品中磺胺类药物残留的非常有用的工具。基于分子印迹聚合物的化学发光传感器用于测定磺胺类药物的图形摘要分析程序。
