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Abstract：
MrgprD, a Mas-related G protein-coupled receptor, is initially identified in sensory neurons of mouse dorsal root ganglia (DRG) and has been suggested to participate in somatosensation. However, MrgprD has recently been found to be expressed outside the nervous system such as in aortic endothelia cells and neutrophils. In this study, we used immunohistochemistry to detect the expression and localization of MrgprD in mouse intestinal tract. The immunoreactivity (IR) of MrgprD was found in the smooth muscle layers of small intestine, colon and rectum. In addition, MrgprD IR was colocalized with F4/80-positive macrophages and CD3-positive T lymphocytes resident in the lamina propria of intestinal mucosa. MrgprD was also found to be expressed in primary peritoneal macrophages and splenic T lymphocytes. Furthermore, the presence of MrgprD mRNA and its protein was detected in murine macrophage-like RAW 264.7 and human T lymphocyte Jurkat cell lines. Our study shows, for the first time, the expression and localization of MrgprD in the intestinal tract and in macrophages and T lymphocytes, indicating the potential roles of MrgprD in intestinal mobility and immunity.
摘要：
MrgprD,Mas相关的G蛋白偶联受体，最初在小鼠背根神经节（DRG）的感觉神经元中鉴定，并已被建议参与体感。然而,最近发现MrgprD在神经系统外表达,例如在主动脉内皮细胞和嗜中性粒细胞中。在这项研究中,我们用免疫组织化学方法检测MrgprD在小鼠肠道中的表达和定位。在小肠平滑肌层中发现MrgprD的免疫反应性(IR),结肠和直肠。此外,MrgprDIR与驻留在肠粘膜固有层中的F4/80阳性巨噬细胞和CD3阳性T淋巴细胞共定位。还发现MrgprD在原代腹膜巨噬细胞和脾T淋巴细胞中表达。此外，在小鼠巨噬细胞样RAW264.7和人T淋巴细胞Jurkat细胞系中检测到MrgprDmRNA及其蛋白的存在。我们的研究表明,第一次,MrgprD在肠道、巨噬细胞和T淋巴细胞中的表达和定位，表明MrgprD在肠道活动和免疫中的潜在作用。
