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Abstract：
Matrix metalloproteinase-2 (MMP2) and matrix metalloproteinase-9 (MMP9) have an important role in the reproductive system and in the fertilisation process. The aim of this study was to investigate the MMP2 and MMP9 activity in semen and their association with the pregnancy rate, semen parameters and seminal plasma oxidative stress parameters in couples who were treated with intrauterine insemination (IUI). The semen specimens were obtained from 60 men who attended with their spouse for the IUI in the infertility unit. A controlled ovarian stimulation was performed with clomiphene citrate in IUI cycles. Women with positive pregnancies were recorded (n = 29). The results showed the activity of sperm MMP2 and seminal plasma MMP9 was significantly higher in the pregnant group, compared to the non-pregnant group (p < .05). There was a correlation between the sperm MMP2 activity and the total thiol group (TTG) (r = 0.276, p < .05) and the total antioxidant capacity (TAC) of seminal plasma (r = 0.304, p < .05). The sperm MMP9 showed a positive correlation with the seminal plasma TAC (r = 0.330, p < .05) and an inverse correlation with the lipid peroxidation (LP) of seminal plasma (r = -304, p< 0.05). In addition, the seminal plasma MMP2 activity was correlated to sperm viability (r = 0.266, p< .05) and the TTG of seminal plasma (r = 0.298, p < .05). The MMP2 activity in the sperm may be an important factor for determining the pregnancy rate after IUI. Impact statement What is already known on this subject? Previous studies have reported that the fusion between the sperm and zona pellucida required the activity of matrix metalloproteinase 2 (MMP2), whereas the inhibition of MMP2 can significantly decrease the in vitro fertilisation (IVF) rate. What do the results of this study add? This study has identified that the sperm MMP2 activity was significantly higher in the pregnant couples in comparison with the non-pregnant couples, who treated with intrauterine insemination (IUI). The findings showed there was a correlation between sperm MMP2 activity and the total thiol group (TTG) and the total antioxidant capacity (TAC) of the seminal plasma. What are the implications of these findings for clinical practice and/or further research? MMP2 activity in the sperm could influence the IUI outcome and it is an important factor for IUI success.
摘要：
基质金属蛋白酶-2（MMP2）和基质金属蛋白酶-9（MMP9）在生殖系统和受精过程中具有重要作用。本研究的目的是调查精液中MMP2和MMP9的活性及其与妊娠率的关系。接受宫腔内人工授精（IUI）治疗的夫妇的精液参数和精浆氧化应激参数。精液标本是从60名与配偶一起参加不育科IUI的男性中获得的。在IUI周期中使用柠檬酸氯米芬进行控制性卵巢刺激。记录了妊娠阳性的妇女（n=29）。成果显示怀胎组精子MMP2和精浆MMP9的活性显著增高，与非妊娠组相比（p<0.05）。精子MMP2活性与精浆总巯基(TTG)(r=0.276,p<.05)和总抗氧化能力(TAC)(r=0.304,p<.05)之间存在相关性。精子MMP9与精浆TAC呈正相关(r=0.330,p<0.05),与精浆脂质过氧化(LP)呈负相关(r=-304,p<0.05)。此外,精浆MMP2活性与精子活力（r=0.266，p<.05）和精浆TTG（r=0.298，p<.05）相关。精子中的MMP2活性可能是决定IUI后妊娠率的重要因素。以前的研究报告说,精子和透明带之间的融合需要基质金属蛋白酶2(MMP2)的活性,而抑制MMP2可以显着降低体外受精（IVF）率。这项研究的结果补充了什么？这项研究发现，与未怀孕的夫妇相比，怀孕夫妇的精子MMP2活性明显更高，谁用宫腔内授精（IUI）治疗。结果表明，精子MMP2活性与精浆的总巯基（TTG）和总抗氧化能力（TAC）之间存在相关性。这些发现对临床实践和/或进一步研究有什么意义？精子中的MMP2活性可能会影响IUI结果，并且是IUI成功的重要因素。
