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Abstract：
The objective of this study is to report a molecularly imprinted polymer-based chemiluminescence method for determination of Sudan dyes. A dummy-template molecularly imprinted polymer capable of recognizing seven Sudan dyes was first synthesized and its recognition mechanism was studied by using computation simulation method. The polymer was coated in the wells of conventional microplate to prepare a chemiluminescence sensor and the assay process consisted of only one sample-loading step prior to signal acquisition. The optimized sensor was used to determine the seven dyes in egg yolk and the results were confirmed with a high performance liquid chromatography. Results showed that this sensor achieved ultrahigh sensitivity (1.0-5.0 pg/mL), rapid assay process (10 min) and satisfactory recovery (70.5%-92.2%). Furthermore, the sensor could be reused for 5 times. Therefore, this sensor could be used as a useful tool for screening the residues of Sudan dyes in egg.
摘要：
本研究的目的是报道一种基于分子印迹聚合物的化学发光法测定苏丹染料的方法。首先合成了一种能够识别7种苏丹红染料的假模板分子印迹聚合物，并利用计算模拟方法研究了其识别机理。将聚合物涂覆在常规微孔板的孔中,以制备化学发光传感器,并且测定过程仅由信号采集之前的一个样品加载步骤组成。优化后的传感器用于测定蛋黄中的7种染料，并通过高效液相色谱法证实了结果。结果表明，该传感器实现了超高灵敏度（1.0-5.0pg/mL），快速测定过程（10分钟）和令人满意的回收率（70.5％-92.2％）。此外，传感器可以重复使用5次。因此,该传感器可用作筛查鸡蛋中苏丹红残留的有用工具。
