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Abstract：
We report a case of healthy live birth from a couple diagnosed with oligoasthenoteratozoospermia (OAT). They failed three ICSI cycles in the past 11 years. In the last cycle, the ovarian stimulation was done with antagonist protocol and six oocytes were aspirated. Semen sample was prepared by one layer gradient. 10 class I spermatozoa were selected by motile-sperm organelle-morphology examination (MSOME) according to Cassuto criteria. The selected spermatozoa were then injected into the MII oocytes. 16-18 h after ICSI, three zygotes was formed and, then, cultured in the time lapse monitoring (TLM) for 3 days. Two best embryos were selected according to the morphokinetic status for transfer. A singleton pregnancy was achieved, resulting in the birth of a healthy baby. This successful outcome shows that use of high technologies of MSOME and TLM procedures are useful for selection of the best spermatozoa and embryos in case of severe male infertility.
摘要：
我们报告了一对被诊断为少弱精子症（OAT）的夫妇的健康活产病例。在过去的11年中，他们失败了三个ICSI周期。在最后一个循环中,用拮抗剂方案进行卵巢刺激,并抽吸6个卵母细胞.通过一层梯度制备精液样品。根据Cassuto标准，通过活动精子细胞器形态学检查（MSOME）选择了10个I类精子。然后将选择的精子注射到MII卵母细胞中。ICSI后16-18小时，形成了三个受精卵，然后,在延时监测(TLM)中培养3天。根据形态动力学状态选择两个最佳胚胎用于转移。单胎怀孕，导致一个健康的婴儿出生。这一成功的结果表明，在严重男性不育的情况下，使用MSOME和TLM程序的高科技可用于选择最佳精子和胚胎。
