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Abstract：
UNASSIGNED: Legionnaires\' disease is an important public health problem that can cause substantial mortality and morbidity. Legionnaires\' disease-risk estimation may be compromised by uncertainties in Legionella-detection methods. The aim of this study was the detection of L. pneumophila in respiratory specimens of hospitalized patients with respiratory symptoms by culture, PCR, and loop-mediated isothermal amplification (LAMP) methods.
UNASSIGNED: Sputum and bronchoalveolar lavage samples were obtained from patients with pneumonia admitted to teaching hospitals in Ahvaz, Iran from June 2016 to March 2017. Isolation of Legionella spp. was done by culturing the samples directly onto buffered charcoal-yeast extract and modified Wadowsky-Yee agar medium. Then, PCR and LAMP assays were performed for detection of L. pneumophila via its mip gene in respiratory specimens.
UNASSIGNED: A total of 100 respiratory specimens were collected. Our results showed that 1% of the samples were culture positive for Legionella spp., and 3% and 7% of samples were positive for L. pneumophila using the mip gene on PCR and LAMP assays, respectively.
UNASSIGNED: Legionnaires\' disease should be considered in the diagnosis of pulmonary infectious diseases. Also, the LAMP assay is a faster method with higher sensitivity and specificity than conventional methods, such as PCR and culture, for laboratory diagnosis of Legionnaires\' disease.
UNASSIGNED: Sputum and bronchoalveolar lavage samples were obtained from patients with pneumonia admitted to teaching hospitals in Ahvaz, Iran from June 2016 to March 2017. Isolation of Legionella spp. was done by culturing the samples directly onto buffered charcoal-yeast extract and modified Wadowsky-Yee agar medium. Then, PCR and LAMP assays were performed for detection of L. pneumophila via its mip gene in respiratory specimens.
UNASSIGNED: A total of 100 respiratory specimens were collected. Our results showed that 1% of the samples were culture positive for Legionella spp., and 3% and 7% of samples were positive for L. pneumophila using the mip gene on PCR and LAMP assays, respectively.
UNASSIGNED: Legionnaires\' disease should be considered in the diagnosis of pulmonary infectious diseases. Also, the LAMP assay is a faster method with higher sensitivity and specificity than conventional methods, such as PCR and culture, for laboratory diagnosis of Legionnaires\' disease.
摘要：
■军团病是一个重要的公共卫生问题，可以导致大量的死亡率和发病率。军团菌检测方法的不确定性可能会损害军团菌疾病风险估计。这项研究的目的是通过培养检测有呼吸道症状的住院患者的呼吸道标本中的肺炎杆菌。PCR,和环介导等温扩增(LAMP)方法。
■痰液和支气管肺泡灌洗样本取自阿瓦士教学医院的肺炎患者，伊朗从2016年6月到2017年3月。军团菌的分离.通过直接在缓冲木炭-酵母提取物和改良的Wadowsky-Yee琼脂培养基上培养样品来完成。然后，进行PCR和LAMP测定，以通过其mip基因在呼吸道标本中检测嗜肺乳杆菌。
■共收集100个呼吸道标本。我们的结果表明，1％的样品对军团菌属呈培养阳性。,3％和7％的样本在PCR和LAMP分析中使用mip基因检测肺炎杆菌阳性，分别。
■在肺部感染性疾病的诊断中应考虑军团病。此外，LAMP测定法是一种比常规方法更快速，具有更高的灵敏度和特异性的方法，如PCR和培养，用于军团病的实验室诊断。
■痰液和支气管肺泡灌洗样本取自阿瓦士教学医院的肺炎患者，伊朗从2016年6月到2017年3月。军团菌的分离.通过直接在缓冲木炭-酵母提取物和改良的Wadowsky-Yee琼脂培养基上培养样品来完成。然后，进行PCR和LAMP测定，以通过其mip基因在呼吸道标本中检测嗜肺乳杆菌。
■共收集100个呼吸道标本。我们的结果表明，1％的样品对军团菌属呈培养阳性。,3％和7％的样本在PCR和LAMP分析中使用mip基因检测肺炎杆菌阳性，分别。
■在肺部感染性疾病的诊断中应考虑军团病。此外，LAMP测定法是一种比常规方法更快速，具有更高的灵敏度和特异性的方法，如PCR和培养，用于军团病的实验室诊断。
