Establishment of an Immortalized Mouse Bmp2 Knockout Dental Papilla Mesenchymal Cell Line.

• 发表时间: 2019
• 来源期刊: Methods Mol Biol
• DOI: 10.1007/978-1-4939-9012-2_2
• PMID: 30838560
• 文章类型: Journal Article
• 作者列表: Xu W,Chen S

关键词: Bmp2 floxed mice; Bone morphogenetic protein 2; Dental papilla mesenchymal cell line; Dentin formation; Dentinogenesis imperfecta; Knockout

Mesh: Animals; Bone Morphogenetic Protein 2 / genetics; Cell Culture Techniques / methods; Cell Line; Dental Papilla / cytology; Mesenchymal Stem Cells; Mice; Mice, Inbred C57BL; Mice, Knockout

Abstract

Bone morphogenetic protein 2 (Bmp2) is essential for dentin formation. Bmp2 cKO mice exhibited similar phenotype to dentinogenesis imperfecta (DGI), showing dental pulp exposure, hypomineralized dentin, and delayed odontoblast differentiation. As it is relatively difficult to obtain primary Bmp2 cKO dental papilla mesenchymal cells and to maintain a long-term culture of these primary cells, availability of immortalized deleted Bmp2 dental papilla mesenchymal cells is critical for studying the underlying mechanism of Bmp2 signal in odontogenesis. Here we describe the generation of an immortalized deleted Bmp2 dental papilla mesenchymal (iBmp2ko/ko-dp) cell line by introducing Cre fluorescent protein (GFP) into the immortalized mouse floxed Bmp2 dental papilla mesenchymal (iBmp2flox/flox-dp) cells.