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Abstract：
OBJECTIVE: Anti-Müllerian Hormone (AMH) concentration is high at birth in males, demonstrating the presence of functional testicular tissue in the prepubertal period, and acting as a useful marker in the investigation of paediatric reproductive disorders. AMH also provides a tool in the investigation of female virilization, premature ovarian failure and polycystic ovarian syndrome in childhood. Robust, assay-specific paediatric AMH reference intervals are therefore required for clinical interpretation of results. The aim of this study was to derive age-specific AMH reference intervals for males and females aged 0-18 years.
METHODS: Plasma samples were obtained from patients at Royal Manchester Children\'s Hospital and analysed for AMH using the automated Beckman Coulter Access AMH Assay. Patients under investigation for paediatric reproductive or endocrine disorders were excluded from the study.
METHODS: Seven hundred and 2 patient plasma samples (465 male, 237 female) were subject to AMH measurement, and results were analysed in order to derive continuous and discrete reference intervals for the paediatric age range.
RESULTS: Clear discrimination between male and female AMH results was evident in the prepubertal age range, with some overlap between the genders following pubertal onset.
CONCLUSIONS: We have derived age-related reference intervals for plasma AMH in the paediatric age range (0-18 years) using the automated Beckman Coulter Access AMH assay which will aid in the investigation of paediatric endocrine disorders such as disorders of sexual development.
METHODS: Plasma samples were obtained from patients at Royal Manchester Children\'s Hospital and analysed for AMH using the automated Beckman Coulter Access AMH Assay. Patients under investigation for paediatric reproductive or endocrine disorders were excluded from the study.
METHODS: Seven hundred and 2 patient plasma samples (465 male, 237 female) were subject to AMH measurement, and results were analysed in order to derive continuous and discrete reference intervals for the paediatric age range.
RESULTS: Clear discrimination between male and female AMH results was evident in the prepubertal age range, with some overlap between the genders following pubertal onset.
CONCLUSIONS: We have derived age-related reference intervals for plasma AMH in the paediatric age range (0-18 years) using the automated Beckman Coulter Access AMH assay which will aid in the investigation of paediatric endocrine disorders such as disorders of sexual development.
摘要：
目的：男性出生时抗苗勒管激素（AMH）浓度很高，证明在青春期前存在功能性睾丸组织，并在儿科生殖障碍的研究中充当有用的标记。AMH还提供了调查女性男性化的工具，儿童时期卵巢早衰和多囊卵巢综合征。健壮,因此,试验特异性儿科AMH参考区间对于结果的临床解释是必需的.这项研究的目的是为0-18岁的男性和女性得出年龄特定的AMH参考间隔。
方法：血浆样本来自皇家曼彻斯特儿童医院的患者，并使用自动BeckmanCoulterAccessAMH分析进行AMH分析。接受儿科生殖或内分泌疾病调查的患者被排除在研究之外。
方法：700和2名患者血浆样本（465名男性，237名女性)接受AMH测量，并对结果进行了分析，以得出儿科年龄范围的连续和离散参考区间。
结果：在青春期前的年龄范围内，男性和女性AMH结果之间有明显的区别。青春期开始后，性别之间有一些重叠。
结论：我们使用自动BeckmanCoulterAccessAMH测定法得出了儿科年龄范围（0-18岁）的血浆AMH的年龄相关参考间隔，这将有助于研究儿科内分泌疾病，例如性发育障碍。
方法：血浆样本来自皇家曼彻斯特儿童医院的患者，并使用自动BeckmanCoulterAccessAMH分析进行AMH分析。接受儿科生殖或内分泌疾病调查的患者被排除在研究之外。
方法：700和2名患者血浆样本（465名男性，237名女性)接受AMH测量，并对结果进行了分析，以得出儿科年龄范围的连续和离散参考区间。
结果：在青春期前的年龄范围内，男性和女性AMH结果之间有明显的区别。青春期开始后，性别之间有一些重叠。
结论：我们使用自动BeckmanCoulterAccessAMH测定法得出了儿科年龄范围（0-18岁）的血浆AMH的年龄相关参考间隔，这将有助于研究儿科内分泌疾病，例如性发育障碍。
