PDF(Pubmed)
Abstract：
UNASSIGNED: Sexual development in Flammulina velutipes is controlled by two different mating type loci (HD and PR). The HD locus contains homeodomain (Hd) genes on two separate HD subloci: HD-a and HD-b. While the functionality of the HD-b sublocus has been largely confirmed, the status and content of the HD-a sublocus has remained unclear.
UNASSIGNED: To examine the function of the HD-a sublocus, genome sequences of a series of F. velutipes strains were analyzed and tested through series of amplification by specific primer sets. Furthermore, activity of di-allelic HD-a locus was confirmed by crossing strains with different combinations of HD-a and HD-b subloci.
UNASSIGNED: Sublocus HD-b contained a large variety of fixed Hd1/Hd2 gene pairs, while the HD-a sublocus either contained a conserved Hd2 gene or, a newly discovered Hd1 gene that was also conserved. Identification of whole HD loci, that is, the contents of HD-a and HD-b subloci in a strain, revealed that strains with similar HD-b subloci could still form normal dikaryons if the two genes at the HD-a sublocus differed. At least di-allelic HD-a sublocus, is thus indicated to be actively involved in mating type compatibility.
UNASSIGNED: HD-a sublocus is active and di-allelic. Using the new information on the HD subloci, primers sets were developed that specifically amplify HD-a or HD-b subloci in the majority of F. velutipes strains. In this way, unknown HD mating types of F. velutipes can now be quickly identified, and HD mating type compatibility conferred by HD-a or HD-b can be confirmed by PCR.
UNASSIGNED: To examine the function of the HD-a sublocus, genome sequences of a series of F. velutipes strains were analyzed and tested through series of amplification by specific primer sets. Furthermore, activity of di-allelic HD-a locus was confirmed by crossing strains with different combinations of HD-a and HD-b subloci.
UNASSIGNED: Sublocus HD-b contained a large variety of fixed Hd1/Hd2 gene pairs, while the HD-a sublocus either contained a conserved Hd2 gene or, a newly discovered Hd1 gene that was also conserved. Identification of whole HD loci, that is, the contents of HD-a and HD-b subloci in a strain, revealed that strains with similar HD-b subloci could still form normal dikaryons if the two genes at the HD-a sublocus differed. At least di-allelic HD-a sublocus, is thus indicated to be actively involved in mating type compatibility.
UNASSIGNED: HD-a sublocus is active and di-allelic. Using the new information on the HD subloci, primers sets were developed that specifically amplify HD-a or HD-b subloci in the majority of F. velutipes strains. In this way, unknown HD mating types of F. velutipes can now be quickly identified, and HD mating type compatibility conferred by HD-a or HD-b can be confirmed by PCR.
摘要：
■金针菇的性发育受两个不同的交配型基因座（HD和PR）控制。HD基因座在两个独立的HD亚基因座HD-a和HD-b上含有同源结构域(Hd)基因。虽然HD-b亚基因座的功能已得到很大程度的证实，HD-a亚基因座的状态和含量尚不清楚.
■要检查HD-a亚基因座的功能，对一系列金针菇菌株的基因组序列进行了分析，并通过一系列的特异性引物扩增进行了测试。此外，通过将菌株与HD-a和HD-b亚基因座的不同组合杂交来确认双等位基因HD-a基因座的活性。
■SublocusHD-b包含多种固定的Hd1/Hd2基因对，而HD-a子基因座要么含有保守的Hd2基因，新发现的Hd1基因也是保守的。整个HD基因座的鉴定，也就是说,菌株中HD-a和HD-b亚基因座的含量，表明，如果HD-a亚基因座上的两个基因不同，则具有相似HD-b亚基因座的菌株仍可以形成正常的二核。至少双等位基因HD-a亚基因座，因此表明积极参与交配型兼容性。
■HD-a亚基因座是活跃的和双等位基因的。利用关于HD亚基因座的新信息，开发了在大多数金针菇菌株中特异性扩增HD-a或HD-b亚基因座的引物组。这样,未知的HD交配类型的金针菇现在可以快速识别，HD-a或HD-b赋予的HD交配型相容性可以通过PCR确认。
■要检查HD-a亚基因座的功能，对一系列金针菇菌株的基因组序列进行了分析，并通过一系列的特异性引物扩增进行了测试。此外，通过将菌株与HD-a和HD-b亚基因座的不同组合杂交来确认双等位基因HD-a基因座的活性。
■SublocusHD-b包含多种固定的Hd1/Hd2基因对，而HD-a子基因座要么含有保守的Hd2基因，新发现的Hd1基因也是保守的。整个HD基因座的鉴定，也就是说,菌株中HD-a和HD-b亚基因座的含量，表明，如果HD-a亚基因座上的两个基因不同，则具有相似HD-b亚基因座的菌株仍可以形成正常的二核。至少双等位基因HD-a亚基因座，因此表明积极参与交配型兼容性。
■HD-a亚基因座是活跃的和双等位基因的。利用关于HD亚基因座的新信息，开发了在大多数金针菇菌株中特异性扩增HD-a或HD-b亚基因座的引物组。这样,未知的HD交配类型的金针菇现在可以快速识别，HD-a或HD-b赋予的HD交配型相容性可以通过PCR确认。
