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Abstract：
Producing heterologous enzymes in the animal digestive tract to improve feed utilization rate is a new research strategy by transgenic technology. In this study, transgenic pigs specifically expressing β-glucanase gene in the intestine were successfully produced by somatic cell nuclear transfer technology in order to improve digestibility of dietary β-glucan and absorption of nutrients. The β-glucanase activity in the intestinal juice of 4 transgenic pigs was found to be 8.59 ± 2.49 U/mL. The feeding trial results showed that the crude protein digestion of 4 transgenic pigs was significantly increased compared with that of the non-transgenic pigs. In order to investigate the inheritance of the transgene, 7 G1 transgenic pigs were successfully obtained. The β-glucanase activity in the intestinal juice of 7 G1 transgenic pigs was found to be 2.35 ± 0.72 U/mL. The feeding trial results showed the crude protein digestion and crude fat digestion were significantly higher in 7 G1 transgenic pigs than in non-transgenic pigs. Taken together, our study demonstrated that the foreign β-glucanase expressing in the intestine of the transgenic pigs could reduce the anti-nutritional effect of β-glucans in feed. In addition, β-glucanase gene could be inherited to the offsprings and maintain its physiological function. It is a promising approach to improve feed utilization by producing transgenic animals.
摘要：
在动物消化道中产生异源酶以提高饲料利用率是转基因技术的新研究策略。在这项研究中,通过体细胞核移植技术成功生产了在肠道中特异性表达β-葡聚糖酶基因的转基因猪，以提高饮食中β-葡聚糖的消化率和营养物质的吸收。发现4只转基因猪的肠液中的β-葡聚糖酶活性为8.59±2.49U/mL。饲喂试验结果表明,4只转基因猪的粗蛋白消化量较非转基因猪显著增加。为了研究转基因的遗传，7只G1转基因猪胜利获得。发现7只G1转基因猪的肠液中的β-葡聚糖酶活性为2.35±0.72U/mL。饲喂试验结果表明，7只G1转基因猪的粗蛋白消化和粗脂肪消化明显高于非转基因猪。一起来看,我们的研究表明，在转基因猪的肠道中表达的外源β-葡聚糖酶可以降低饲料中β-葡聚糖的抗营养作用。此外,β-葡聚糖酶基因可遗传给后代,维持其生理功能。通过生产转基因动物来提高饲料利用率是一种有前途的方法。
