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Abstract：
The aim of this study was to compare the expression of p63 protein and calponin in terms of their affinity and specificity for myoepithelial cells in canine mammary tumours. The studied material included 10 benign and 32 malignant mammary tumours from female dogs treated with mastectomy. Primary mouse monoclonal antibodies directed against p63 protein clone 4A4 and calponin clone CALP were used in single- and doublestain system of immunohistochemical reaction. The investigations have shown that majority of myoepithelial cells in benign tumours and carcinomas in situ exhibited strong positive labelling for both markers. In other malignant tumours strong immunoreactivity was observed in resting myoepithelial cells (MECs) and hypertrophic myoepithelial cells (HMECs), while the immunoreactivity in spindle-stellate myoepithelial cells (SMECs) and rounded myoepithelial cells (RMECs) was moderate. The granular-diffuse nuclear expression of p63 protein was observed only in myoepithelial cells. In terms of calponin, diffuse cytoplasmic expression was noted not only in myoepithelial cell but also in some stromal fibroblasts and vascular smooth muscle cells. The epithelial cells did not exhibit specific expression of the investigated markers. The obtained results indicate that p63 is a sensitive and more specific marker of myoepithelial cells in canine mammary tumours compared with calponin. These findings suggest that the immunohistochemical analysis peformed with the use of p63 can be a valuable complement of routine histological examinations of canine mammary tumours facilitating identification of tumours with myoepithelial component.
摘要：
这项研究的目的是比较p63蛋白和钙蛋白对犬乳腺肿瘤中肌上皮细胞的亲和力和特异性的表达。研究的材料包括10例良性和32例恶性乳腺肿瘤，这些肿瘤来自接受乳房切除术治疗的雌性犬。针对p63蛋白克隆4A4和钙蛋白克隆CALP的原代小鼠单克隆抗体用于免疫组织化学反应的单染色和双染色系统。研究表明，良性肿瘤和原位癌中的大多数肌上皮细胞对两种标志物均表现出强烈的阳性标记。在其他恶性肿瘤中，在静息肌上皮细胞（MEC）和肥大肌上皮细胞（HMEC）中观察到强烈的免疫反应性，而梭形星状肌上皮细胞（SMEC）和圆形肌上皮细胞（RMEC）的免疫反应性中等。仅在肌上皮细胞中观察到p63蛋白的颗粒扩散核表达。就Calponin而言,不仅在肌上皮细胞中，而且在一些基质成纤维细胞和血管平滑肌细胞中也注意到弥漫性细胞质表达。上皮细胞未显示所研究标记物的特异性表达。获得的结果表明，与calponin相比，p63是犬乳腺肿瘤中肌上皮细胞的敏感且更特异的标记。这些发现表明，使用p63进行的免疫组织化学分析可能是犬乳腺肿瘤常规组织学检查的有价值的补充，有助于鉴定具有肌上皮成分的肿瘤。
