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Abstract：
Ginsenoside compound K (CK) is not a ginsenoside that naturally exists in Panax ginseng Meyer. However, CK is a major metabolite of ginsenoside Rb1, Rb2, or Rc in the intestine under the effects of bacteria. In this study, we first investigated the effects of CK on myelosuppression in mice induced by cyclophosphamide (CTX). The respective quantities of white blood cells, blood platelets, and bone marrow nucleated cells (BMNCs) were determined to be 8.54 ± 0.91 (109/L), 850.90 ± 44.11 (109/L), and 1.45 ± 0.22 (109/L) in the CK-H group by detecting peripheral blood cells and BMNCs. CK-H and CK-L both increased the thymus index by up to 0.62 ± 0.06 (mg/g) and 0.52 ± 0.09 (mg/g), respectively, and significantly increased the yields of colony formation units-granulocyte monocyte and colony formation units-megakaryocytic. According to our study, CK could control apoptosis and promote cells to enter the normal cell cycle by the bcl-2/bax signaling pathway and MEK/ERK signaling pathway. Therefore, the BMNCs could proliferate and differentiate normally after entering the normal cell cycle. So the peripheral blood cells could show a trend of returning to normal. The recovery of peripheral blood cells resulting in the level of cytokines tended to normal. This process may be the mechanisms of CK on myelosuppression. This study provides a reference for ginseng in the treatment of myelosuppression.
摘要：
人参皂苷化合物K(CK)不是人参中天然存在的人参皂苷。然而,CK是人参皂苷Rb1、Rb2或Rc在肠道细菌作用下的主要代谢产物。在这项研究中,我们首先研究了CK对环磷酰胺（CTX）诱导的小鼠骨髓抑制的影响。白细胞的数量,血小板,和骨髓有核细胞（BMNCs）测定为8.54±0.91（109/L），850.90±44.11(109/L),通过检测外周血细胞和BMNCs，CK-H组为1.45±0.22（109/L）。CK-H和CK-L均使胸腺指数增加了0.62±0.06（mg/g）和0.52±0.09（mg/g），分别,并显着增加了集落形成单位-粒细胞单核细胞和集落形成单位-巨核细胞的产量。根据我们的研究,CK可通过bcl-2/bax信号通路和MEK/ERK信号通路控制细胞凋亡,促进细胞进入正常细胞周期。因此,进入正常细胞周期后，BMNCs可以正常增殖和分化。因此，外周血细胞可以显示出恢复正常的趋势。外周血细胞恢复导致细胞因子水平趋于正常。这一过程可能是CK对骨髓抑制的机制。本研究为人参治疗骨髓抑制提供参考。
