PDF(Sci-hub)
Abstract：
A case of suspected acute and lethal intoxication caused by colchicine has been reported. The woman was hospitalized after her suspicion of suicidal poisoning by a rare autumn crocus (Colchicum autumnale). Suspected colchicine poisoning was confirmed using a novel UHPLC method with a modern reversed-phase stationary phase with a sub 2-micron superficial porous particle size combined with a QTOF mass spectrometer. Sample preparation procedure included the addition of propiverine as internal standard, protein precipitation using methanol and solid phase extraction. High-resolution MS only and targeted MS/MS modes are reported for the qualitative analysis and screening of other potential drugs of abuse in blood samples. All Ion MS mode was used for quantitative determination of colchicine afterward. The concentration of colchicine in the blood sample was approximately 41 ng/mL, and more than 200 μg/mL of the plant extract used for the suicide.
摘要：
已报道一例由秋水仙碱引起的疑似急性和致命性中毒。该名女子因怀疑罕见的秋番红花（秋葵）自杀中毒而住院。使用新型UHPLC方法，并结合QTOF质谱仪，使用具有亚2微米表面多孔粒径的现代反相固定相，确认了可疑的秋水仙碱中毒。样品制备程序包括添加丙哌维林作为内标，蛋白质沉淀使用甲醇和固相萃取。报告了仅高分辨率MS和靶向MS/MS模式，用于定性分析和筛选血液样品中其他潜在滥用药物。之后使用所有离子MS模式定量测定秋水仙碱。血液样品中秋水仙碱的浓度约为41ng/mL，和超过200μg/mL的用于自杀的植物提取物。
