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Abstract：
Although, Patulin and Ochratoxin are produced by the same genera of molds, however, Patulin was the most extensively studied mycotoxins in apple juice and no reports have explored the presence of Ochratoxin A in the apple juice. Therefore, the objective of this study was to explore the presence of Patulin and Ochratoxin A in apple juice in Saudi Arabian market of Jeddah. Potato dextrose agar(PDA) was used to detect fungal contamination. Patulin was determined using HPLC equipped with a UV detector set at 276 nm. Also, HPLC with fluorescence detector was set at 333 and 420 nm as excitation and emission wavelength, respectively,was used for Ochratoxin A separation. All samples of apple juice were free from fungi and yeasts. The Patulin (PAT) was detected in only one type out of 17 types (5.88%) with a concentration of 152.5 ppb, (305%) increased compared with the maximum permitted level (50 ppb). However the occurrence of Ochratoxin A (OTA) in apple juice samples was discovered in 5 types out of 17 types (29.41%). The concentration of OTA ranged from 100 to 200 ppb reaching 5-10-folds compared with the permissible limits (20 ppb).
摘要：
虽然,棒曲霉素和曲霉毒素是由相同属的霉菌产生的，然而,棒曲霉素是苹果汁中研究最广泛的霉菌毒素，尚无报道探讨苹果汁中是否存在OchratoxinA。因此,这项研究的目的是探索沙特阿拉伯吉达市场苹果汁中的Patulin和OchratoxinA的存在。马铃薯葡萄糖琼脂（PDA）用于检测真菌污染。使用配备有设定在276nm的UV检测器的HPLC测定棒曲霉素。此外，将具有荧光检测器的HPLC设定在333和420nm作为激发和发射波长，分别,用于曲霉毒素A的分离。所有苹果汁样品均不含真菌和酵母。在浓度为152.5ppb的17种类型（5.88％）中，仅检测到一种类型的Patulin（PAT），（305％）与最大允许水平（50ppb）相比增加。然而，在17种类型中的5种类型（29.41％）中发现了苹果汁样品中的OchratoxinA（OTA）。OTA的浓度范围为100至200ppb，与允许的限值（20ppb）相比，达到5-10倍。
