Abstract：
BACKGROUND: The pathogenesis and treatment of cutaneous malodour in dogs have not been investigated previously. Staphylococcus and Corynebacterium spp. are associated with human axillary malodour.
OBJECTIVE: Staphylococcus and Corynebacterium spp. are associated with cutaneous malodour in dogs, and treatment with a topical essential oil-based product will improve malodour and reduce the abundance of odour-causing bacteria.
METHODS: Twenty seven bloodhound dogs from a south Texas boarding facility were enrolled in this study.
METHODS: Skin swabs were taken from the axilla and dorsum of 27 dogs at initiation of the study. Mean malodour scores were used to assign dogs to control or malodour groups. The malodourous dogs were randomly assigned to a treatment or placebo group, received four weekly topical applications of the spot-on or placebo, and samples were recollected. Next-generation sequencing (NGS) and real-time quantitative PCR (qPCR) were performed on all swabs.
RESULTS: Psychrobacter and Pseudomonas spp. were significantly more abundant (P < 0.001, P = 0.006; respectively), and overall bacterial diversity was reduced (P = 0.0384) on the skin of malodourous dogs. Staphylococcus and Corynebacterium spp. were not associated with malodour. The topical essential oil-based product significantly (P = 0.0078) improved malodour in the treatment group and shifted their bacterial community structure.
CONCLUSIONS: A novel association of bacterial genera with malodour in bloodhound dogs, identified by NGS, highlights future targets for odour control. The topical treatment significantly reduced malodour. The interaction between the topical treatment and cutaneous microbiota should be further investigated and may be useful in other dermatological conditions involving microbiota.
OBJECTIVE: Staphylococcus and Corynebacterium spp. are associated with cutaneous malodour in dogs, and treatment with a topical essential oil-based product will improve malodour and reduce the abundance of odour-causing bacteria.
METHODS: Twenty seven bloodhound dogs from a south Texas boarding facility were enrolled in this study.
METHODS: Skin swabs were taken from the axilla and dorsum of 27 dogs at initiation of the study. Mean malodour scores were used to assign dogs to control or malodour groups. The malodourous dogs were randomly assigned to a treatment or placebo group, received four weekly topical applications of the spot-on or placebo, and samples were recollected. Next-generation sequencing (NGS) and real-time quantitative PCR (qPCR) were performed on all swabs.
RESULTS: Psychrobacter and Pseudomonas spp. were significantly more abundant (P < 0.001, P = 0.006; respectively), and overall bacterial diversity was reduced (P = 0.0384) on the skin of malodourous dogs. Staphylococcus and Corynebacterium spp. were not associated with malodour. The topical essential oil-based product significantly (P = 0.0078) improved malodour in the treatment group and shifted their bacterial community structure.
CONCLUSIONS: A novel association of bacterial genera with malodour in bloodhound dogs, identified by NGS, highlights future targets for odour control. The topical treatment significantly reduced malodour. The interaction between the topical treatment and cutaneous microbiota should be further investigated and may be useful in other dermatological conditions involving microbiota.
摘要：
背景：以前尚未研究狗皮肤恶臭的发病机理和治疗方法。葡萄球菌和棒状杆菌属。与人类腋臭有关.
目的：葡萄球菌和棒状杆菌属。与狗的皮肤恶臭有关，和治疗与局部精油为基础的产品将改善恶臭和减少大量的气味引起的细菌。
方法：这项研究招募了来自德克萨斯州南部寄宿设施的27只猎犬。
方法:在研究开始时从27只狗的腋下和背部取皮肤拭子。平均恶臭评分用于将狗分配到对照组或恶臭组。恶毒的狗被随机分配到治疗组或安慰剂组，接受了四次每周局部应用的现场或安慰剂，并收集了样本。对所有拭子进行下一代测序(NGS)和实时定量PCR(qPCR)。
结果：嗜冷杆菌和假单胞菌属。更丰富（P<0.001，P=0.006；分别），和整体细菌多样性减少（P=0.0384）的狗的皮肤。葡萄球菌和棒状杆菌属。与恶臭无关。局部精油基产品显着改善了治疗组的恶臭（P=0.0078），并改变了其细菌群落结构。
结论：嗜血犬中细菌属与恶臭的新关联，由NGS识别，突出了气味控制的未来目标。局部治疗显著减少了恶臭。应进一步研究局部治疗与皮肤微生物群之间的相互作用，并可能用于其他涉及微生物群的皮肤病学状况。
目的：葡萄球菌和棒状杆菌属。与狗的皮肤恶臭有关，和治疗与局部精油为基础的产品将改善恶臭和减少大量的气味引起的细菌。
方法：这项研究招募了来自德克萨斯州南部寄宿设施的27只猎犬。
方法:在研究开始时从27只狗的腋下和背部取皮肤拭子。平均恶臭评分用于将狗分配到对照组或恶臭组。恶毒的狗被随机分配到治疗组或安慰剂组，接受了四次每周局部应用的现场或安慰剂，并收集了样本。对所有拭子进行下一代测序(NGS)和实时定量PCR(qPCR)。
结果：嗜冷杆菌和假单胞菌属。更丰富（P<0.001，P=0.006；分别），和整体细菌多样性减少（P=0.0384）的狗的皮肤。葡萄球菌和棒状杆菌属。与恶臭无关。局部精油基产品显着改善了治疗组的恶臭（P=0.0078），并改变了其细菌群落结构。
结论：嗜血犬中细菌属与恶臭的新关联，由NGS识别，突出了气味控制的未来目标。局部治疗显著减少了恶臭。应进一步研究局部治疗与皮肤微生物群之间的相互作用，并可能用于其他涉及微生物群的皮肤病学状况。
