Abstract：
In order to provide an efficient way to degrade carboxymethylcellulose (CMC), three pathways were investigated: enzymolysis, combination of ultrasound pretreatment and enzymolysis, and sonoenzymolysis. Effects of these treatments on enzymatic kinetics, degradation kinetics and properties of degraded CMC were investigated. The degradation degree of CMC was increased by 18.90% and 35.73% with ultrasound pretreatment (at an intensity of 24 W/mL for 30 min) and sonoenzymolysis (at an intensity of 9 W/mL for 50 min), compared with that obtained under the traditional enzymolysis. Analysis of kinetics demonstrated that ultrasound, both pretreatment and combined with β-glucanase, could accelerate CMC degradation. Measurements of rheological properties, molecular weight and structures of CMC hydrolysates revealed that ultrasound broke the glycosidic bond of CMC chains without changing its primary structure. The sonoenzymolysis process was the most efficient method to degrade CMC, with potential to provide a way to obtain CMC with lowest molecular weight or viscosity.
摘要：
为了提供一种高效的降解羧甲基纤维素(CMC)的方法,研究了三种途径：酶解，超声波预处理和酶解相结合，和声酶解。这些处理对酶动力学的影响，研究了降解CMC的降解动力学和降解性能。经超声预处理(强度为24W/mL,30min)和超声酶解(强度为9W/mL,50min),CMC的降解程度分别提高了18.90%和35.73%,与传统酶解下获得的相比。动力学分析表明，超声，预处理和结合β-葡聚糖酶，可以加速CMC降解。流变特性的测量，CMC水解产物的分子量和结构表明，超声破坏了CMC链的糖苷键，而不改变其一级结构。超声酶解工艺是降解CMC最有效的方法，具有提供获得具有最低分子量或粘度的CMC的方法的潜力。
