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Abstract：
This research was aimed to explore correlation of gene polymorphisms of CD36 and ApoE with susceptibility of Alzheimer disease (AD).This study was a case-control study. Two hundred eleven AD hospitalized patients were selected as the AD group and 241 subjects were selected as the control group. PCR-RFLP was used to detect three loci (rs7755, rs3211956, and rs10499859) of CD36 gene and ApoE genotype. Chi-square test and univariate nonconditional logistic regression analysis were used to calculate the odds ratio (OR) and 95% confidence interval (95% CI). The haplotypes were constructed using SHEsis online software and the correlation between haplotypes and AD was analyzed. Meanwhile, differences of 3 alleles of ApoE and 6 genotypes (E2/E2, E2/E3, E2/E4, E3/E3, E3/E4, E4/E4) were compared between AD and control groups.The frequencies of rs7755 genotype (χ = 10.780, P = .005) and allele (χ = 10.549, P = .001) were statistically different between 2 groups. The genotype frequency of rs3211956 was statistically different between AD and control groups (χ = 10.119, P = .006). For the rs7755 locus, GG genotype (OR: 2.013, 95% CI: 1.098-3.699) was an independent risk factor for AD compared with AA genotype. In the dominant model, the risk to develop AD in AG/GG genotype was 1.686 times higher than AA genotype. For the rs3211956 locus, compared with TT genotype, GT genotype (OR: 0.536, 95% CI: 0.340-0.846) was a protective factor for AD after adjusting various physiological and biochemical factors. In the dominant model, the risk of GT/GG genotype to develop AD was reduced by 41.6%. For ApoE gene, the distribution differences of E2/E3 (χ = 9.216, P = .002), E3/E4 (χ = 7.728, P = .005), and E4/E4 had statistical significance between the 2 groups. The frequencies of allele E2 (χ = 9.359, P = .002) and E4 (χ = 13.995, P < .001) were statistically significant between AD and control groups.The rs7755 and rs3211956 loci polymorphisms of CD36 gene and genotype E2/E3, E3/E4, E4/E4 of ApoE gene, and E2 and E4 alleles were statistically related with AD.
摘要：
本研究旨在探讨CD36和ApoE基因多态性与阿尔茨海默病(AD)易感性的相关性。本研究为病例对照研究。选择210例AD住院患者作为AD组，并选择241例受试者作为对照组。用PCR-RFLP检测CD36基因和ApoE基因型的三个位点(rs7755、rs3211956和rs10499859)。采用卡方检验和单变量非条件逻辑回归分析计算比值比(OR)和95%置信区间(95%CI)。利用SHEsis在线软件构建单倍型,分析单倍型与AD的相关性。同时,比较了AD组和对照组之间ApoE的3个等位基因和6种基因型（E2/E2，E2/E3，E2/E4，E3/E3，E3/E4，E4/E4）的差异。rs7755基因型(χ=10.780,P=.005)和等位基因(χ=10.549,P=.001)频率在两组间差异有统计学意义。rs3211956基因型频率在AD组和对照组间差异有统计学意义(χ=10.119,P=.006)。对于rs7755基因座，与AA基因型相比,GG基因型(OR:2.013,95%CI:1.098~3.699)是AD的独立危险因素。在主导模型中，AG/GG基因型发生AD的风险是AA基因型的1.686倍.对于rs3211956基因座，与TT基因型相比，GT基因型(OR:0.536,95%CI:0.340～0.846)是调节多种生理生化因素后发生AD的保护因素。在主导模型中，GT/GG基因型发生AD的风险降低了41.6%.对于ApoE基因,E2/E3的分布差异(χ=9.216，P=.002)，E3/E4（χ=7.728，P=0.005），E4/E4在两组间有统计学意义。E2等位基因频率(χ=9.359,P=.002)和E4等位基因频率(χ=13.995,P<.001)在AD组和对照组间差异有统计学意义。CD36基因rs7755和rs3211956位点多态性与ApoE基因E2/E3、E3/E4、E4/E4基因型,E2和E4等位基因与AD有统计学相干性。
