关键词: Affinity tag Expression Fusion protein Purification R9AP RGS9-1 anchor protein Solubility Solubility-enhancing tag
Mesh : Adaptor Proteins, Signal Transducing / biosynthesis genetics isolation & purification Base Sequence Biotechnology / methods Chromatography, Affinity / methods Cloning, Molecular Escherichia coli / genetics metabolism Gene Expression Genetic Vectors / chemistry metabolism Glutathione Transferase / genetics isolation & purification metabolism Histidine / genetics isolation & purification metabolism Humans Maltose-Binding Proteins / genetics isolation & purification metabolism Membrane Proteins / biosynthesis genetics isolation & purification Oligopeptides / genetics isolation & purification metabolism Protein Engineering / methods Recombinant Fusion Proteins / biosynthesis genetics isolation & purification Solubility
来 源: DOI:10.1016/j.pep.2018.07.007