序列 C 端色氨酸对绿色荧光蛋白荧光的影响。Effect of sequential C-terminal tryptophans on green fluorescent protein fluorescence.-医云文献数字医云科研云海量医学决策数据服务

Abstract：

The effect of the addition of sequential C-terminal tryptophan residues on the fluorescence intensity of GFP was investigated. Tandem repeats of six tryptophan residues markedly decreased fluorescence intensity. This phenomenon is likely to occur because of the inhibition of GFP folding, resulting in insolubility. Exploiting this phenomenon, we constructed a cloning vector that facilitates the identification of recombinant colonies of Escherichia coli by the activation of GFP.

摘要：

研究了连续C末端色氨酸残基的添加对GFP荧光强度的影响。六个色氨酸残基的串联重复显着降低了荧光强度。由于GFP折叠的抑制，这种现象很可能发生，导致不溶性。利用这种现象，我们构建了一个克隆载体，通过激活GFP促进大肠杆菌重组菌落的鉴定。