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Abstract：
The aim of this case study is to show how traditional and molecular methods can be employed to identify the Mugilidae species currently used in Sardinia (Italy) to produce the traditional bottarga for the processing of their ovaries. A total of six specimens of Mugil cephalus (n=3) and Mugil capurrii (n=3) were subjected to external morphology and meristic measurements. Subsequently, tissue samples of white muscle and ovaries from three individuals per species were underwent PCR-sequencing assay of mitochondrial DNA cytochrome oxidase subunit I (COI). The external morphology and meristic characters showed a sufficient level of reliability in the identification between the two species. At the same time, the molecular techniques showed the discriminatory power and confirmed the correct species identification in all the sampling units. DNA barcoding may be an effective aid to traditional taxonomy and can facilitate accurate species identification among the Mugilidae.
摘要：
本案例研究的目的是展示如何采用传统和分子方法来鉴定撒丁岛（意大利）目前使用的Mugilidae物种，以生产用于卵巢加工的传统bottarga。对总共六个Mugilcepharus（n=3）和Mugilcapurrii（n=3）的标本进行了外部形态和遗传学测量。随后，对每个物种3个个体的白肌肉和卵巢组织样本进行线粒体DNA细胞色素氧化酶亚基I(COI)的PCR测序分析.外部形态和分生特征在两个物种之间的鉴定中显示出足够的可靠性。同时,分子技术显示了辨别能力，并在所有采样单元中确认了正确的物种识别。DNA条形码可能是对传统分类学的有效帮助，并且可以促进Mugilidae之间的准确物种鉴定。
