Abstract：
The success of pregnancy depends on the maternal immune system\'s ability to promote tolerance and host defense. This equilibrium is compromised in inflammatory and infectious impairment of placenta. Smoking during pregnancy exposes the fetus to severe complications which might result from an alteration in placenta macrophages (pMφ) functions. In this study, we assessed the effect of cigarette smoke extract (CSE) on the functions of third trimester pMφs.CSE inhibited particles uptake and the formation of multinucleated giant cells, a recently reported property of pMφs based on their ability to fuse in vitro. These alterations were associated with a CSE-induced abnormal activation of pMφs, which was characterized by an increased release of TNF, interleukin (IL)-33, and decreased IL-6 and IL-10 release. Furthermore, CSE enhanced the expression of metalloproteinase genes known to be involved in tissue remodeling. This effect of CSE on pMφs was specific because CSE affected circulating monocytes in a different way. Finally, we showed that nicotine affected in part the functional properties of pMφs. Taken together, these results showed that CSE modulated the functional activity of pMφs, which may compromise pregnancy.
摘要：
妊娠的成功取决于母体免疫系统促进耐受和宿主防御的能力。这种平衡在胎盘的炎症和感染性损伤中受损。怀孕期间吸烟会使胎儿遭受严重的并发症，这可能是由于胎盘巨噬细胞（pMφ）功能的改变所致。在这项研究中,我们评估了香烟烟雾提取物（CSE）对妊娠晚期pMφs功能的影响。CSE抑制颗粒摄取和多核巨细胞的形成，最近报道的pMφs的特性是基于它们在体外融合的能力。这些改变与CSE诱导的pMφs异常激活有关，其特征是TNF释放增加，白细胞介素（IL）-33，并减少IL-6和IL-10的释放。此外，CSE增强了已知参与组织重塑的金属蛋白酶基因的表达。CSE对pMφs的这种作用是特异性的,因为CSE以不同的方式影响循环单核细胞。最后,我们表明尼古丁部分影响pMφs的功能特性。一起来看,这些结果表明，CSE调节了pMφs的功能活性，这可能会影响怀孕。
