关键词: clone library denaturing gradient gel electrophoresis meta-omics molecular ecological networks pure culture pyrosequencing stable isotope probing

来  源:   DOI:10.3389/fmicb.2018.00520   PDF(Pubmed)

Abstract:
The Deepwater Horizon (DWH) oil spill in the Gulf of Mexico in 2010 resulted in serious damage to local marine and coastal environments. In addition to the physical removal and chemical dispersion of spilled oil, biodegradation by indigenous microorganisms was regarded as the most effective way for cleaning up residual oil. Different microbiological methods were applied to investigate the changes and responses of bacterial communities after the DWH oil spills. By summarizing and analyzing these microbiological methods, giving recommendations and proposing some methods that have not been used, this review aims to provide constructive guidelines for microbiological studies after environmental disasters, especially those involving organic pollutants.
摘要:
2010年墨西哥湾深水地平线(DWH)漏油事件对当地海洋和沿海环境造成了严重破坏。除了物理去除和化学分散溢油之外,本地微生物的生物降解被认为是清除残留油的最有效方法。采用不同的微生物学方法研究了DWH溢油后细菌群落的变化和反应。通过总结和分析这些微生物方法,提出建议并提出一些尚未使用的方法,这篇综述旨在为环境灾害后的微生物研究提供建设性的指导方针,尤其是那些涉及有机污染物的。
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