Consensus recommendations for trabecular meshwork cell isolation, characterization and culture.

小梁细胞分离的共识建议 ，表征和文化。

• 影响指数: 3.77
• 发表时间: 06 2018
• 来源期刊: Exp Eye Res
• DOI: 10.1016/j.exer.2018.03.001
• PMID: 29526795
• 文章类型: Journal Article
• 中科院分区: Q1
• 方向: 医学
• 作者列表: Keller KE,Bhattacharya SK,Borrás T,Brunner TM,Chansangpetch S,Clark AF,Dismuke WM,Du Y,Elliott MH,Ethier CR,Faralli JA,Freddo TF,Fuchshofer R,Giovingo M,Gong H,Gonzalez P,Huang A,Johnstone MA,Kaufman PL,Kelley MJ,Knepper PA,Kopczynski CC,Kuchtey JG,Kuchtey RW,Kuehn MH,Lieberman RL,Lin SC,Liton P,Liu Y,Lütjen-Drecoll E,Mao W,Masis-Solano M,McDonnell F,McDowell CM,Overby DR,Pattabiraman PP,Raghunathan VK,Rao PV,Rhee DJ,Chowdhury UR,Russell P,Samples JR,Schwartz D,Stubbs EB,Tamm ER,Tan JC,Toris CB,Torrejon KY,Vranka JA,Wirtz MK,Yorio T,Zhang J,Zode GS,Fautsch MP,Peters DM,Acott TS,Stamer WD

关键词: Aqueous humor dynamics; Conventional outflow; Glaucoma; Intraocular pressure; Ocular hypertension

Mesh: Age Factors; Animals; Biomarkers / metabolism; Cell Culture Techniques; Cell Separation / methods; Consensus; Fetus; Guidelines as Topic; Humans; Tissue Donors; Tissue Preservation; Tissue and Organ Harvesting; Trabecular Meshwork / cytology metabolism

Abstract

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

摘要

培养的小梁网（TM）细胞是一种有价值的模型系统，可用于研究与调节常规流出阻力和眼内压有关的细胞机制；以及它们的功能障碍导致高眼压。在这次审查中,我们描述了用于从包括人类在内的几种动物物种中分离TM细胞的标准程序，以及用于验证其身份的方法。拥有一套TM细胞的标准实践将增加科学的严谨性，当用作模型时，并使其他研究人员能够复制和建立以前的发现。