关键词: Affinity constant (K(d)) Competitive displacement analysis Fluorometric ligand binding detection Förster resonance energy transfer (FRET) Phosphodiesterase 5 cGMPS-rhodamine
Mesh : Catalytic Domain Chemistry, Pharmaceutical / methods Cyclic GMP / chemistry Cyclic Nucleotide Phosphodiesterases, Type 5 / chemistry metabolism Fluorescence Resonance Energy Transfer / methods Fluorescent Dyes / chemistry Humans Isoenzymes / chemistry metabolism Ligands Models, Chemical Molecular Docking Simulation Phosphodiesterase 5 Inhibitors / pharmacology Protein Binding Recombinant Proteins / chemistry metabolism Rhodamines / chemistry Tadalafil / pharmacology
来 源:
DOI:10.1016/j.jpba.2017.11.014
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