关键词: DNA barcoding Mullidae cryptic species mediterranean sea red sea

来  源:   DOI:10.3923/pjbs.2016.65.70

Abstract:
Despite ongoing efforts to protect species and ecosystems in the egyptian costs of the Red Sea and Mediterranean Sea, habitat degradation, overfishing and pollution have posed serious challenges to marine natural resources. In spite of the accumulated knowledge on the systematics of commercial fishes in Egypt recent results suggested that we are far from having a complete picture of egyptian fish diversity. The accurate identification of species is a very important component in many fields of biological research and conservation efforts. The high level of expertise and time consuming process needed means a loss in biodiversity. Successful species identification is now frequently based on a combination of approaches including morphometrics and the sequencing of the mitochondrial COI gene known as the DNA barcoding. This study employed COI sequencing alongside traditional taxonomic identification methods and uncovered cryptic diversity within the goat fish species of Family Mullidae, four species collected from both the Red Sea and the Mediterranean Sea. Upeneus pori, Upeneus vittatus, Mullus surmuletus and Mullus barbatus samples from the Red Sea and the Mediterranean were clustered separately in a NJ tree indicating the possibility of the presence of cryptic species complex. All species could be differentiated by their COI sequence.
摘要:
尽管一直在努力保护红海和地中海的埃及物种和生态系统,栖息地退化,过度捕捞和污染对海洋自然资源构成了严峻挑战。尽管积累了有关埃及商业鱼类系统学的知识,但最近的结果表明,我们还远远没有对埃及鱼类多样性的完整了解。物种的准确识别是生物学研究和保护工作的许多领域中非常重要的组成部分。所需的高水平专业知识和耗时的过程意味着生物多样性的丧失。现在,成功的物种鉴定通常基于多种方法的组合,包括形态计量学和称为DNA条形码的线粒体COI基因的测序。这项研究采用了COI测序以及传统的分类学鉴定方法,并揭示了Mullidae家族的山羊鱼类物种中的隐秘多样性,从红海和地中海收集的四个物种。Upeneuspori,维塔图斯,来自红海和地中海的Mullussurmuletus和Mullusbarbatus样品分别聚集在NJ树中,表明存在隐秘物种复合体的可能性。所有物种都可以通过其COI序列来区分。
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