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Abstract：
BACKGROUND: In this study, we compare glycosylation at the fetomaternal interface in 3 equine species: horse, donkey and zebra, all of which can interbreed to produce hybrids, to assess their glycan similarities and differences.
METHODS: Sections cut from 3 specimens of horse (Equus caballus) placenta (50, 200 and 280 days gestation), one donkey (Equus asinus) placenta (65 cm crown-rump length) and 5 specimens of zebra (Equus quagga) placentae (81-239 days gestation) were stained with a panel of 24 biotinylated lectins using an avidin-peroxidase revealing system.
RESULTS: There were only slight quantitative differences in the lectin histochemistry at the feto-maternal interface between all three specimens; zebra placentae expressed more α2,6-linked sialic acid, with α1,2fucosyl residues at the microvillous interface. However, zebra trophoblast showed histological differences from the other two species, with polarised cells, prominent supranuclear Golgi bodies, and fewer intracellular granules.
CONCLUSIONS: Our findings appear to confirm the hypothesis that closely related, interbreeding species with epitheliochorial placentae express similar glycans at the feto-maternal interface, thereby supporting the existence of a placental glycocode. We also observed intraspecies evolutionary diversion to be associated with a different histological architecture and the absence of significant intracellular granules.
METHODS: Sections cut from 3 specimens of horse (Equus caballus) placenta (50, 200 and 280 days gestation), one donkey (Equus asinus) placenta (65 cm crown-rump length) and 5 specimens of zebra (Equus quagga) placentae (81-239 days gestation) were stained with a panel of 24 biotinylated lectins using an avidin-peroxidase revealing system.
RESULTS: There were only slight quantitative differences in the lectin histochemistry at the feto-maternal interface between all three specimens; zebra placentae expressed more α2,6-linked sialic acid, with α1,2fucosyl residues at the microvillous interface. However, zebra trophoblast showed histological differences from the other two species, with polarised cells, prominent supranuclear Golgi bodies, and fewer intracellular granules.
CONCLUSIONS: Our findings appear to confirm the hypothesis that closely related, interbreeding species with epitheliochorial placentae express similar glycans at the feto-maternal interface, thereby supporting the existence of a placental glycocode. We also observed intraspecies evolutionary diversion to be associated with a different histological architecture and the absence of significant intracellular granules.
摘要：
背景：在这项研究中，我们比较了3种马的母胎界面的糖基化：马，驴和斑马,所有这些都可以杂交产生杂种，评估它们的聚糖相似性和差异。
方法：从3个马（马）胎盘标本（妊娠50、200和280天）切下的切片，使用抗生物素蛋白-过氧化物酶揭示系统，用一组24种生物素化的凝集素对一头驴（马牛）胎盘（胎冠长度65厘米）和5个斑马（马牛）胎盘标本（妊娠81-239天）进行染色。
结果：所有三个标本之间的胎儿-母体界面处的凝集素组织化学仅存在微小的定量差异；斑马胎盘表达更多的α2,6连接的唾液酸，在微绒毛界面处具有α1,2岩藻糖基残基。然而,斑马滋养层显示出与其他两个物种的组织学差异，极化的细胞，突出的超核高尔基体，细胞内颗粒较少。
结论：我们的发现似乎证实了密切相关的假设，与上皮视胎盘的杂交物种在胎儿-母体界面表达相似的聚糖，从而支持胎盘糖编码的存在。我们还观察到种内进化转移与不同的组织学结构和缺乏明显的细胞内颗粒有关。
方法：从3个马（马）胎盘标本（妊娠50、200和280天）切下的切片，使用抗生物素蛋白-过氧化物酶揭示系统，用一组24种生物素化的凝集素对一头驴（马牛）胎盘（胎冠长度65厘米）和5个斑马（马牛）胎盘标本（妊娠81-239天）进行染色。
结果：所有三个标本之间的胎儿-母体界面处的凝集素组织化学仅存在微小的定量差异；斑马胎盘表达更多的α2,6连接的唾液酸，在微绒毛界面处具有α1,2岩藻糖基残基。然而,斑马滋养层显示出与其他两个物种的组织学差异，极化的细胞，突出的超核高尔基体，细胞内颗粒较少。
结论：我们的发现似乎证实了密切相关的假设，与上皮视胎盘的杂交物种在胎儿-母体界面表达相似的聚糖，从而支持胎盘糖编码的存在。我们还观察到种内进化转移与不同的组织学结构和缺乏明显的细胞内颗粒有关。
