The immune-enhancing activity of Cervus nippon mantchuricus extract (NGE) in RAW264.7 macrophage cells and immunosuppressed mice.

• 影响指数: 7.425
• 发表时间: 09 2017
• 来源期刊: Food Res Int
• DOI: 10.1016/j.foodres.2017.06.053
• PMID: 28784525
• 文章类型: Journal Article
• 中科院分区: Q1
• 作者列表: Hong SH,Ku JM,In Kim H,Ahn CW,Park SH,Seo HS,Shin YC,Ko SG

关键词: Cervus nippon mantchuricus Extract; Cyclophosphamide (PubChem CID: 2907); Deer bone; Immune-enhancing activity; Immunosuppressed mice; Lipopolysaccharide (PubChem CID: 11970143); MAPK; Macrophages; NF-κB

Mesh: Animals; Blotting, Western; Bone and Bones / immunology; Cell Culture Techniques; Cell Differentiation / drug effects immunology; Cell Survival / drug effects immunology; Cytokines / drug effects immunology; Deer; Enzyme-Linked Immunosorbent Assay; Immunologic Factors / immunology; Immunosuppression Therapy; Macrophages / drug effects immunology; Male; Medicine, Korean Traditional / methods; Mice; Mice, Inbred BALB C; Mitogen-Activated Protein Kinases / drug effects immunology; Models, Animal; NF-kappa B / drug effects immunology; Nitric Oxide / immunology; RAW 264.7 Cells; Signal Transduction / drug effects immunology; Tissue Extracts / immunology

Abstract

Chemotherapeutics are often used to inhibit the proliferation of cancer cells. However, they can also harm healthy cells and cause side effects such as immunosuppression. Especially traditional oriental medicines long used in Asia, may be beneficial candidates for the alleviation of immune diseases. Cervus nippon mantchuricus extract (NGE) is currently sold in the market as coffee and health drinks. However, NGE was not widely investigated and efficacy remain unclear and essentially nothing is known about their potential immune-regulatory properties. As a result, NGE induced the differentiation of RAW264.7 macrophage cells. NGE-stimulated RAW264.7 macrophage cells elevated cytokines levels and NO production. NGE-stimulated RAW264.7 macrophage cells activated MAPKs and NF-κB signaling pathways. NGE encouraged the immuno-enhancing effects in immunosuppressed short-term treated with NGE mice model. NGE or Red ginseng encouraged the immuno-enhancing effects in immunosuppressed long-term treated with NGE mice model. Our data clearly show that NGE contains immune-enhancing activity and can be used to treat immunodeficiency.