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Abstract：
Some enzyme supplement products claim benefits for healthy dogs to compensate for alleged suboptimal production of endogenous enzymes and the loss of enzymes in commercial pet foods secondary to processing. The objective of the current study was to determine macronutrient and energy digestibility by healthy adult dogs fed a commercial maintenance diet with or without supplementation with plant- and animal-origin enzyme products at the dosage recommended by their respective manufacturers. A group of fourteen healthy neutered adult Beagle dogs (average age 8 years) was divided into two equal groups and fed the basal diet alone and then with either the plant- or animal-origin enzyme supplement in three consecutive 10-d periods; the treatment groups received the opposite enzyme supplement in the third period. Digestibility in each period was performed by the total faecal collection method. Serum trypsin-like immunoreactivity (TLI) was measured at the end of each trial. Data were analysed by repeated measures and the α level of significance was set at 0·05. There were no differences in energy and nutrient digestibility between enzyme treatments. When comparing basal with enzyme supplementation, fat digestibility was higher for the basal diet compared with the animal-origin enzyme treatment, which could be a period effect and was not biologically significant (94·7 v. 93·5 %). Serum TLI was not affected by supplementation with either enzyme product. Exogenous enzyme supplementation did not significantly increase digestibility of a typical commercial dry diet in healthy adult dogs and routine use of such products is not recommended.
摘要：
一些酶补充产品声称对健康狗有益，以补偿所谓的内源性酶的次优生产和加工后商业宠物食品中酶的损失。当前研究的目的是确定健康成年犬的大量营养素和能量消化率，该成年犬饲喂商业维持饮食，以各自制造商推荐的剂量补充或不补充植物和动物来源的酶产品。将一组14只健康的成年比格犬（平均年龄8岁）分为两个相等的组，分别单独喂养基础饮食，然后在三个连续10天的时间内补充植物或动物来源的酶；治疗组在第三阶段接受相反的酶补充剂。通过总粪便收集方法进行每个时期的消化率。在每个试验结束时测量血清胰蛋白酶样免疫反应性(TLI)。通过重复测量分析数据，显著性α水平设定为0·05。酶处理之间的能量和养分消化率没有差异。当比较基础和酶补充剂时，与动物来源的酶处理相比，基础饮食的脂肪消化率更高，这可能是一个时期的影响，并没有生物学意义（94·7v93·5%）。血清TLI不受补充任一种酶产品的影响。在健康成年犬中，补充外源酶不会显着增加典型商业干饮食的消化率，因此不建议常规使用此类产品。
