Abstract：
Two polysaccharides, GCP-I-I and GCP-II-I, were obtained from 100°C water extracts of Gentiana crassicaulis roots by DEAE anion exchange chromatography and gel filtration. The results from methanolysis, methylation, FT-IR and NMR, indicated that these two fractions are typical pectic polysaccharides, with HG and RG-I regions and AG-I/AG-II side chains, and some of the galacturonic acid units of fraction GCP-I-I were methyl esterified. Fractions GCP-I-I and GCP-II-I, both exhibited potent complement fixation, and fraction GCP-I-I was more potent than positive control BPII. The higher complement fixation activity obtained in fraction GCP-I-I may be due to the higher Mw and/or higher amount of AG-II present in fraction GCP-I-I than fraction GCP-II-I. The polysaccharides from G. crassicaulis could be used as a potential natural immunomodulator.
摘要：
两种多糖，GCP-I-I和GCP-II-I,通过DEAE阴离子交换色谱和凝胶过滤从龙胆的100°C水提取物中获得。甲醇分解的结果，甲基化,FT-IR和NMR,表明这两个部分是典型的果胶多糖，具有HG和RG-I区以及AG-I/AG-II侧链，和部分GCP-I-I的一些半乳糖醛酸单元被甲酯化。馏分GCP-I-I和GCP-II-I,两者都表现出有效的补体固定，和部分GCP-I-I比阳性对照BPII更有效。在级分GCP-I-I中获得的更高的补体固定活性可能是由于级分GCP-I-I中存在的AG-II的Mw和/或更高的量高于级分GCP-II-I。粗甘草多糖可作为一种潜在的天然免疫调节剂。
