Abstract：
G antigen of Rh blood group system is present either along with D and/or C positive red cells. Hence, [serologically anti-G presents with the similar picture as that of multiple antibodies (anti-D + anti-C). Differentiating them is important as anti-D + anti-C causes severe hemolytic disease of the fetus and newborn than anti-G. In pregnancies with anti-G alone, alloimmunization due to D antigen could be prevented by prophylactic administration of RhIg. Differentiating between anti-D + C from anti-G in alloimmunized pregnant mothers becomes essential. Sera from antenatal mothers, whose antibody identification by 11-cell panel gave a pattern for anti-D and anti-C were selected. Extended phenotyping for Rh system was performed for these antenatal cases. Differential adsorption and elution testing using R2R2 cells initially and r\'r cells subsequently were performed to distinguish anit-G from anti-D + anti-C. Antibody titers of these antibodies were determined and their clinical outcome in the newborn was followed. A pattern suggestive of anti D and anti C on antibody identification were observed in six antenatal cases. On further workup 50 % of them confirmed to have anti G. Antibody titers of anti-G and anti-C were lower than that of Anti-D. All newborns were sensitized in vivo and the antibody specificity in them were confirmed with elution studies. The mothers who had only anti-G were subsequently administered with an appropriate dose of RhIg.Differential adsorption and elution studies help in identifying anti-G and distinguishing it from anti-D plus anti-C, thus helping in better patient management.
摘要：
Rh血型系统的G抗原与D和/或C阳性红细胞一起存在。因此，[血清学抗-G呈现与多种抗体(抗-D+抗-C)相似的图像。区分它们很重要，因为抗D+抗C比抗G会导致胎儿和新生儿严重的溶血病。在单独使用抗G的怀孕中，通过预防性施用RhIg可以预防由于D抗原引起的同种免疫。在同种免疫妊娠母亲中区分抗DC和抗G变得至关重要。产前母亲的血清，选择了通过11个细胞小组鉴定的抗体，从而得出了抗D和抗C的模式。对这些产前病例进行了Rh系统的扩展表型鉴定。最初使用R2R2细胞和随后使用r'r细胞进行差异吸附和洗脱测试，以区分anit-G和抗D+抗C。确定这些抗体的抗体滴度，并跟踪其在新生儿中的临床结果。在6例产前病例中观察到提示抗D和抗C抗体鉴定的模式。在进一步的检查中，其中50％证实具有抗G。抗G和抗C的抗体滴度低于抗D。所有新生儿均在体内致敏，并通过洗脱研究证实了其中的抗体特异性。仅具有抗G的母亲随后施用适当剂量的RhIg。差异吸附和洗脱研究有助于识别抗G，并将其与抗D+抗C区分开，从而有助于更好的患者管理。
