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Abstract：
An electrochemical genosensor based on Zinc oxide/platinum-palladium (ZnO/Pt-Pd) modified fluorine doped tin oxide (FTO) glass plate was fabricated for detection of consensus DNA sequence of Dengue virus (DENV) using methylene blue (MB) as an intercalating agent. To achieve it, probe DNA (PDNA) was immobilized on the surface of ZnO/Pt-Pd nanocomposites modified FTO electrode. The synthesized nano-composites were characterized by high resolution transmission electron microscopy (HRTEM), energy dispersive X-ray analysis (EDX), atomic force microscopy (AFM), scanning electron microscopy (SEM), UV-Vis spectroscopy, X-ray diffraction (XRD) analysis and Fourier transform infra-red (FTIR) spectroscopy. This PDNA modified electrode (PDNA/ZnO/Pt-Pd/FTO) served as a signal amplification platform for the detection of the target hybridized DNA (TDNA). The hybridization between PDNA and TDNA was detected by reduction in current, generated by interaction of anionic mediator, i.e., methylene blue (MB) with free guanine (3\'G) of ssDNA. The sensor showed a dynamic linear range of 1 × 10-6M to 100 × 10-6M with LOD as 4.3 × 10-5 M and LOQ as 9.5 × 10-5 M. Till date, majorly serotype specific biosensors for dengue detection have been developed. The genosensor reported here eliminates the possibility of false result as in case of serotype specific DNA sensor. This is the report where conserved sequences present in all the serotypes of Dengue virus has been employed for fabrication of a genosensor.
摘要：
以亚甲基蓝（MB）为嵌入剂，制备了基于氧化锌/铂-钯（ZnO/Pt-Pd）修饰的氟掺杂氧化锡（FTO）玻璃板的电化学基因传感器，用于检测登革热病毒（DENV）的共有DNA序列。为了实现它，将探针DNA（PDNA）固定在ZnO/Pt-Pd纳米复合材料修饰的FTO电极表面。通过高分辨率透射电子显微镜(HRTEM)对合成的纳米复合材料进行了表征,能量色散X射线分析（EDX），原子力显微镜（AFM），扫描电子显微镜（SEM），紫外-可见光谱,X射线衍射(XRD)分析和傅里叶变换红外(FTIR)光谱。该PDNA修饰电极(PDNA/ZnO/Pt-Pd/FTO)用作检测靶杂交DNA(TDNA)的信号放大平台。通过降低电流检测PDNA和TDNA之间的杂交，由阴离子介质的相互作用产生，即，亚甲基蓝（MB）与ssDNA的游离鸟嘌呤（3'G）。传感器的动态线性范围为1×10-6M至100×10-6M，LOD为4.3×10-5M，LOQ为9.5×10-5M。已经开发了用于登革热检测的主要血清型特异性生物传感器。此处报道的基因传感器消除了血清型特异性DNA传感器的情况下错误结果的可能性。这是其中存在于登革热病毒的所有血清型中的保守序列已用于制造基因传感器的报道。
