Abstract：
A number of animal models have been developed to examine the pathophysiological consequences of surgical procedures, but anesthetic methods, monitoring, and management measures in these models are very different from those used in humans. This study was designed to create a rat model of abdominal surgery using anesthetic methods and perioperative treatment similar to those used in the clinic and to investigate the effects of different injury severities and depths of anesthesia and analgesia on surgical stress and postoperative recovery. Abdominal skin/muscle incision was compared with exploratory laparotomy in rats under propofol intravenous anesthesia, accompanied by perioperative measures such as oxygen inhalation, fluid infusion, warmth, blood gas analysis, and infection prevention. Stress indices (mean arterial pressure, heart rate, blood glucose, and plasma corticosterone) were monitored during anesthesia and surgery, and recovery indicators (body weight, food consumption, and pain) were measured after surgery. In addition, animals undergoing laparotomy were subjected to low and high dosages of propofol and sufentanil, in order to examine the relationship between anesthetic and analgesic depth and stress on recovery. Exploratory laparotomy induced a greater stress response and caused slower postoperative recovery as measured than somatic injury. High-dose sufentanil downregulated plasma corticosterone and improved postoperative recovery more effectively than high-dose propofol (P<0.05). Taken together, a rat model of abdominal surgery using anesthetic methods and perioperative treatment similar to those used in the clinic was successfully developed. It showed a positive correlation between severity of surgical trauma and stress response and postoperative recovery and a significant role of adequate analgesia in reducing surgical stress and improving postoperative recovery.
摘要：
已经开发了许多动物模型来检查外科手术的病理生理后果。但麻醉方法,监测,这些模型中的管理措施与人类使用的措施有很大不同。本研究旨在使用麻醉方法和类似于临床的围手术期处理方法建立大鼠腹部手术模型，并研究不同损伤严重程度和麻醉镇痛深度对手术应激和术后恢复的影响。丙泊酚静脉麻醉下腹部皮肤/肌肉切口与剖腹探查术比较,伴随着围手术期的措施，如吸氧，输液,温暖,血气分析,和感染预防。压力指数(平均动脉压，心率,血糖，和血浆皮质酮）在麻醉和手术期间进行监测，和恢复指标(体重，食物消费,和疼痛）在手术后测量。此外,接受剖腹手术的动物接受低剂量和高剂量的丙泊酚和舒芬太尼，为了研究麻醉和镇痛深度与恢复压力之间的关系。与躯体损伤相比，剖腹探查术引起的应激反应更大，术后恢复较慢。高剂量舒芬太尼较高剂量异丙酚能显著降低患者血浆皮质酮水平,改善术后恢复(P<0.05)。一起来看,使用麻醉方法和类似于临床的围手术期治疗，成功建立了大鼠腹部手术模型。显示手术创伤严重程度与应激反应和术后恢复呈正相关，足量镇痛在减轻手术应激和改善术后恢复方面具有显著作用。
